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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Sheep Myoglobin (MB) CSB-YP013529SH
CSB-EP013529SH
CSB-BP013529SH
CSB-MP013529SH
CSB-EP013529SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Histone H2A.V (H2AFV) CSB-YP010095CH
CSB-EP010095CH
CSB-BP010095CH
CSB-MP010095CH
CSB-EP010095CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Non-histone chromosomal protein HMG-17 (HMGN2) CSB-YP010570BO
CSB-EP010570BO
CSB-BP010570BO
CSB-MP010570BO
CSB-EP010570BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Non-histone chromosomal protein HMG-14 (HMGN1) CSB-YP010568BO
CSB-EP010568BO
CSB-BP010568BO
CSB-MP010568BO
CSB-EP010568BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Sperm protamine P1 (Prm1) CSB-YP018728MO
CSB-EP018728MO
CSB-BP018728MO
CSB-MP018728MO
CSB-EP018728MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Keratin-associated protein 8-1 (KRTAP8-1) CSB-YP012680SH
CSB-EP012680SH
CSB-BP012680SH
CSB-MP012680SH
CSB-EP012680SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Collagen alpha-1 (II) chain (COL2A1), partial CSB-YP005739HU
CSB-EP005739HU
CSB-BP005739HU
CSB-MP005739HU
CSB-EP005739HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Collagen alpha-1 (IV) chain (COL4A1), partial CSB-YP005741HU
CSB-BP005741HU
CSB-MP005741HU
CSB-EP005741HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Collagen alpha-2 (I) chain (COL1A2), partial CSB-YP005728BO
CSB-EP005728BO
CSB-BP005728BO
CSB-MP005728BO
CSB-EP005728BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Laminin subunit gamma-1 (Lamc1), partial CSB-YP012735MO
CSB-EP012735MO
CSB-BP012735MO
CSB-MP012735MO
CSB-EP012735MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-crystallin A chain (CRYAA) CSB-YP006007HU
CSB-EP006007HU
CSB-BP006007HU
CSB-MP006007HU
CSB-EP006007HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Alpha-crystallin B chain (CRYAB) CSB-YP006008HU
CSB-BP006008HU
CSB-MP006008HU
CSB-EP006008HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Beta-crystallin B1 (Crybb1) CSB-YP006012RA
CSB-EP006012RA
CSB-BP006012RA
CSB-MP006012RA
CSB-EP006012RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Keratin, type I cytoskeletal 10 (Krt10) CSB-YP012504MO
CSB-EP012504MO
CSB-BP012504MO
CSB-MP012504MO
CSB-EP012504MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Vimentin (VIM) CSB-YP025857PI
CSB-EP025857PI
CSB-BP025857PI
CSB-MP025857PI
CSB-EP025857PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Neurofilament light polypeptide (NEFL), partial CSB-YP015688PI
CSB-EP015688PI
CSB-BP015688PI
CSB-MP015688PI
CSB-EP015688PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Spectrin alpha chain, erythrocyte (SPTA1), partial CSB-YP022632HU
CSB-EP022632HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit Troponin C, skeletal muscle (TNNC2) CSB-YP024010RB
CSB-EP024010RB
CSB-BP024010RB
CSB-MP024010RB
CSB-EP024010RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Troponin C, skeletal muscle (TNNC2) CSB-YP024010CH
CSB-EP024010CH
CSB-BP024010CH
CSB-MP024010CH
CSB-EP024010CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Myosin light polypeptide 6 (MYL6) CSB-YP015313CH
CSB-EP015313CH
CSB-BP015313CH
CSB-MP015313CH
CSB-EP015313CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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