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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pneumoniae serotype 19F Regulatory protein recX (recX) CSB-YP477146FNE
CSB-EP477146FNE
CSB-BP477146FNE
CSB-MP477146FNE
CSB-EP477146FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Chaperone protein DnaK (dnaK), partial CSB-YP477147FNE
CSB-EP477147FNE
CSB-BP477147FNE
CSB-MP477147FNE
CSB-EP477147FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP477148FNE
CSB-EP477148FNE
CSB-BP477148FNE
CSB-MP477148FNE
CSB-EP477148FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP477149FNE
CSB-EP477149FNE
CSB-BP477149FNE
CSB-MP477149FNE
CSB-EP477149FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Ribonuclease P protein component (rnpA) CSB-YP477150FNE
CSB-EP477150FNE
CSB-BP477150FNE
CSB-MP477150FNE
CSB-EP477150FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Hydroxyethylthiazole kinase 1 (thiM1) CSB-YP477151FNE
CSB-EP477151FNE
CSB-BP477151FNE
CSB-MP477151FNE
CSB-EP477151FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP477152FNE
CSB-EP477152FNE
CSB-BP477152FNE
CSB-MP477152FNE
CSB-EP477152FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 30S ribosomal protein S20 (rpsT) CSB-YP477153FNE
CSB-EP477153FNE
CSB-BP477153FNE
CSB-MP477153FNE
CSB-EP477153FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Xaa-Pro dipeptidyl-peptidase (pepX), partial CSB-YP477154FNE
CSB-EP477154FNE
CSB-BP477154FNE
CSB-MP477154FNE
CSB-EP477154FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Gamma-glutamyl phosphate reductase (proA) CSB-YP477155FNE
CSB-EP477155FNE
CSB-BP477155FNE
CSB-MP477155FNE
CSB-EP477155FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP477156FNE
CSB-EP477156FNE
CSB-BP477156FNE
CSB-MP477156FNE
CSB-EP477156FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F SsrA-binding protein (smpB) CSB-YP477157FNE
CSB-EP477157FNE
CSB-BP477157FNE
CSB-MP477157FNE
CSB-EP477157FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP477158FNE
CSB-EP477158FNE
CSB-BP477158FNE
CSB-MP477158FNE
CSB-EP477158FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Glycogen synthase (glgA) CSB-YP477159FNE
CSB-EP477159FNE
CSB-BP477159FNE
CSB-MP477159FNE
CSB-EP477159FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F ATP-dependent helicase/deoxyribonuclease subunit B (rexB), partial CSB-YP477160FNE
CSB-EP477160FNE
CSB-BP477160FNE
CSB-MP477160FNE
CSB-EP477160FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP477161FNE
CSB-EP477161FNE
CSB-BP477161FNE
CSB-MP477161FNE
CSB-EP477161FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F Bifunctional protein pyrR (pyrR) CSB-YP477162FNE
CSB-EP477162FNE
CSB-BP477162FNE
CSB-MP477162FNE
CSB-EP477162FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F tRNA (Ile)-lysidine synthase CSB-YP477163FNE
CSB-EP477163FNE
CSB-BP477163FNE
CSB-MP477163FNE
CSB-EP477163FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP477164FNE
CSB-EP477164FNE
CSB-BP477164FNE
CSB-MP477164FNE
CSB-EP477164FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae serotype 19F HPr kinase/phosphorylase (hprK) CSB-YP477165FNE
CSB-EP477165FNE
CSB-BP477165FNE
CSB-MP477165FNE
CSB-EP477165FNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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