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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Klebsiella pneumoniae Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP477746KBH
CSB-EP477746KBH
CSB-BP477746KBH
CSB-MP477746KBH
CSB-EP477746KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Electron transport complex protein RnfA (rnfA), partial CSB-YP477747KBH1
CSB-EP477747KBH1
CSB-BP477747KBH1
CSB-MP477747KBH1
CSB-EP477747KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Coenzyme PQQ synthesis protein E (pqqE) CSB-YP477748KBH
CSB-EP477748KBH
CSB-BP477748KBH
CSB-MP477748KBH
CSB-EP477748KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphate acyltransferase (plsX) CSB-YP477749KBH
CSB-EP477749KBH
CSB-BP477749KBH
CSB-MP477749KBH
CSB-EP477749KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae O-acetyl-ADP-ribose deacetylase (ymdB) CSB-YP477750KBH
CSB-EP477750KBH
CSB-BP477750KBH
CSB-MP477750KBH
CSB-EP477750KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae FMN reductase (NADH) RutF CSB-YP477751KBH
CSB-EP477751KBH
CSB-BP477751KBH
CSB-MP477751KBH
CSB-EP477751KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Chorismate--pyruvate lyase (ubiC) CSB-YP477752KBH
CSB-EP477752KBH
CSB-BP477752KBH
CSB-MP477752KBH
CSB-EP477752KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP477753KBH
CSB-EP477753KBH
CSB-BP477753KBH
CSB-MP477753KBH
CSB-EP477753KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Serine--tRNA ligase (serS) CSB-YP477754KBH
CSB-EP477754KBH
CSB-BP477754KBH
CSB-MP477754KBH
CSB-EP477754KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP477755KBH
CSB-EP477755KBH
CSB-BP477755KBH
CSB-MP477755KBH
CSB-EP477755KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Phosphomethylpyrimidine synthase (thiC), partial CSB-YP477756KBH
CSB-EP477756KBH
CSB-BP477756KBH
CSB-MP477756KBH
CSB-EP477756KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 50S ribosomal protein L11 (rplK) CSB-YP477757KBH
CSB-EP477757KBH
CSB-BP477757KBH
CSB-MP477757KBH
CSB-EP477757KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (ubiD) CSB-YP477758KBH
CSB-EP477758KBH
CSB-BP477758KBH
CSB-MP477758KBH
CSB-EP477758KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ribosomal protein S6 modification protein (rimK) CSB-YP477759KBH
CSB-EP477759KBH
CSB-BP477759KBH
CSB-MP477759KBH
CSB-EP477759KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Ketol-acid reductoisomerase (ilvC) CSB-YP477760KBH
CSB-EP477760KBH
CSB-BP477760KBH
CSB-MP477760KBH
CSB-EP477760KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Met repressor (metJ) CSB-YP477761KBH
CSB-EP477761KBH
CSB-BP477761KBH
CSB-MP477761KBH
CSB-EP477761KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Cation-efflux pump FieF (fieF), partial CSB-YP477762KBH1
CSB-EP477762KBH1
CSB-BP477762KBH1
CSB-MP477762KBH1
CSB-EP477762KBH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Rhamnulose-1-phosphate aldolase (rhaD) CSB-YP477763KBH
CSB-EP477763KBH
CSB-BP477763KBH
CSB-MP477763KBH
CSB-EP477763KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Formimidoylglutamase (hutG) CSB-YP477764KBH
CSB-EP477764KBH
CSB-BP477764KBH
CSB-MP477764KBH
CSB-EP477764KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Endonuclease 8 (nei) CSB-YP477765KBH
CSB-EP477765KBH
CSB-BP477765KBH
CSB-MP477765KBH
CSB-EP477765KBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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