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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vibrio fischeri Probable intracellular septation protein A (VFMJ11_1108), partial CSB-YP477346VFC1
CSB-EP477346VFC1
CSB-BP477346VFC1
CSB-MP477346VFC1
CSB-EP477346VFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Elongation factor P-like protein (VFMJ11_1350) CSB-YP477347VFC
CSB-EP477347VFC
CSB-BP477347VFC
CSB-MP477347VFC
CSB-EP477347VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP477348VFC
CSB-EP477348VFC
CSB-BP477348VFC
CSB-MP477348VFC
CSB-EP477348VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP477349VFC
CSB-EP477349VFC
CSB-BP477349VFC
CSB-MP477349VFC
CSB-EP477349VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphomethylpyrimidine synthase (thiC), partial CSB-YP477350VFC
CSB-EP477350VFC
CSB-BP477350VFC
CSB-MP477350VFC
CSB-EP477350VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri ATP-dependent RNA helicase rhlB (rhlB) CSB-YP477351VFC
CSB-EP477351VFC
CSB-BP477351VFC
CSB-MP477351VFC
CSB-EP477351VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribonuclease PH (rph) CSB-YP477352VFC
CSB-EP477352VFC
CSB-BP477352VFC
CSB-MP477352VFC
CSB-EP477352VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP477353VFC
CSB-EP477353VFC
CSB-BP477353VFC
CSB-MP477353VFC
CSB-EP477353VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP477354VFC
CSB-EP477354VFC
CSB-BP477354VFC
CSB-MP477354VFC
CSB-EP477354VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Tyrosine--tRNA ligase (tyrS) CSB-YP477355VFC
CSB-EP477355VFC
CSB-BP477355VFC
CSB-MP477355VFC
CSB-EP477355VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S12 (rpsL) CSB-YP477356VFC
CSB-EP477356VFC
CSB-BP477356VFC
CSB-MP477356VFC
CSB-EP477356VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S19 (rpsS) CSB-YP477357VFC
CSB-EP477357VFC
CSB-BP477357VFC
CSB-MP477357VFC
CSB-EP477357VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 30S ribosomal protein S14 (rpsN) CSB-YP477358VFC
CSB-EP477358VFC
CSB-BP477358VFC
CSB-MP477358VFC
CSB-EP477358VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L32 (rpmF) CSB-YP477359VFC
CSB-EP477359VFC
CSB-BP477359VFC
CSB-MP477359VFC
CSB-EP477359VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Integration host factor subunit beta (ihfB) CSB-YP477360VFC
CSB-EP477360VFC
CSB-BP477360VFC
CSB-MP477360VFC
CSB-EP477360VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Nitric oxide reductase FlRd-NAD (+) reductase CSB-YP477361VFC
CSB-EP477361VFC
CSB-BP477361VFC
CSB-MP477361VFC
CSB-EP477361VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L15 (rplO) CSB-YP477362VFC
CSB-EP477362VFC
CSB-BP477362VFC
CSB-MP477362VFC
CSB-EP477362VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 50S ribosomal protein L21 (rplU) CSB-YP477363VFC
CSB-EP477363VFC
CSB-BP477363VFC
CSB-MP477363VFC
CSB-EP477363VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP477364VFC
CSB-EP477364VFC
CSB-BP477364VFC
CSB-MP477364VFC
CSB-EP477364VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Glutamate--tRNA ligase (gltX) CSB-YP477365VFC
CSB-EP477365VFC
CSB-BP477365VFC
CSB-MP477365VFC
CSB-EP477365VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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