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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidithiobacillus ferrooxidans Orotate phosphoribosyltransferase (pyrE) CSB-YP477226AWI
CSB-EP477226AWI
CSB-BP477226AWI
CSB-MP477226AWI
CSB-EP477226AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans UPF0234 protein Lferr_1091 (Lferr_1091) CSB-YP477227AWI
CSB-EP477227AWI
CSB-BP477227AWI
CSB-MP477227AWI
CSB-EP477227AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP477228AWI
CSB-EP477228AWI
CSB-BP477228AWI
CSB-MP477228AWI
CSB-EP477228AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans DNA repair protein recO (recO) CSB-YP477229AWI
CSB-EP477229AWI
CSB-BP477229AWI
CSB-MP477229AWI
CSB-EP477229AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP477230AWI
CSB-EP477230AWI
CSB-BP477230AWI
CSB-MP477230AWI
CSB-EP477230AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP477231AWI
CSB-EP477231AWI
CSB-BP477231AWI
CSB-MP477231AWI
CSB-EP477231AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Phosphoglycerate kinase (pgk) CSB-YP477232AWI
CSB-EP477232AWI
CSB-BP477232AWI
CSB-MP477232AWI
CSB-EP477232AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Probable phosphatase VFMJ11_A0091 (VFMJ11_A0091) CSB-YP477233VFC
CSB-EP477233VFC
CSB-BP477233VFC
CSB-MP477233VFC
CSB-EP477233VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Biotin synthase (bioB) CSB-YP477234VFC
CSB-EP477234VFC
CSB-BP477234VFC
CSB-MP477234VFC
CSB-EP477234VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP477235VFC
CSB-EP477235VFC
CSB-BP477235VFC
CSB-MP477235VFC
CSB-EP477235VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Anti-adapter protein iraP (iraP) CSB-YP477236SWK
CSB-EP477236SWK
CSB-BP477236SWK
CSB-MP477236SWK
CSB-EP477236SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP477237SWK
CSB-EP477237SWK
CSB-BP477237SWK
CSB-MP477237SWK
CSB-EP477237SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP477238SWK
CSB-EP477238SWK
CSB-BP477238SWK
CSB-MP477238SWK
CSB-EP477238SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L28 (rpmB) CSB-YP477239SWK
CSB-EP477239SWK
CSB-BP477239SWK
CSB-MP477239SWK
CSB-EP477239SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Phosphonoacetaldehyde hydrolase (phnX) CSB-YP477240SWK
CSB-EP477240SWK
CSB-BP477240SWK
CSB-MP477240SWK
CSB-EP477240SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona HMP-PP phosphatase (cof) CSB-YP477241SWK
CSB-EP477241SWK
CSB-BP477241SWK
CSB-MP477241SWK
CSB-EP477241SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona DNA replication and repair protein recF (recF) CSB-YP477242SWK
CSB-EP477242SWK
CSB-BP477242SWK
CSB-MP477242SWK
CSB-EP477242SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 50S ribosomal protein L25 (rplY) CSB-YP477243SWK
CSB-EP477243SWK
CSB-BP477243SWK
CSB-MP477243SWK
CSB-EP477243SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Chromosomal replication initiator protein DnaA (dnaA) CSB-YP477244SWK
CSB-EP477244SWK
CSB-BP477244SWK
CSB-MP477244SWK
CSB-EP477244SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona ATP synthase epsilon chain (atpC) CSB-YP477245SWK
CSB-EP477245SWK
CSB-BP477245SWK
CSB-MP477245SWK
CSB-EP477245SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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