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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 High frequency lysogenization protein HflD (hflD) CSB-YP477886EOE
CSB-EP477886EOE
CSB-BP477886EOE
CSB-MP477886EOE
CSB-EP477886EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Periplasmic nitrate reductase (napA) CSB-YP477887EOE
CSB-EP477887EOE
CSB-BP477887EOE
CSB-MP477887EOE
CSB-EP477887EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Lipopolysaccharide core heptose (II)-phosphate phosphatase CSB-YP477888EOE
CSB-EP477888EOE
CSB-BP477888EOE
CSB-MP477888EOE
CSB-EP477888EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Chromosomal replication initiator protein DnaA (dnaA) CSB-YP477889EOE
CSB-EP477889EOE
CSB-BP477889EOE
CSB-MP477889EOE
CSB-EP477889EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Membrane protein insertase YidC (yidC), partial CSB-YP477890EOE1
CSB-EP477890EOE1
CSB-BP477890EOE1
CSB-MP477890EOE1
CSB-EP477890EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cell volume regulation protein A (cvrA), partial CSB-YP477891EOE
CSB-EP477891EOE
CSB-BP477891EOE
CSB-MP477891EOE
CSB-EP477891EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP477892EOE
CSB-EP477892EOE
CSB-BP477892EOE
CSB-MP477892EOE
CSB-EP477892EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 o-succinylbenzoate synthase (menC) CSB-YP477893EOE
CSB-EP477893EOE
CSB-BP477893EOE
CSB-MP477893EOE
CSB-EP477893EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0208 membrane protein YfbV (yfbV), partial CSB-YP477894EOE1
CSB-EP477894EOE1
CSB-BP477894EOE1
CSB-MP477894EOE1
CSB-EP477894EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein viaA (viaA) CSB-YP477895EOE
CSB-EP477895EOE
CSB-BP477895EOE
CSB-MP477895EOE
CSB-EP477895EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 4-alpha-L-fucosyltransferase (wecF) CSB-YP477896EOE
CSB-EP477896EOE
CSB-BP477896EOE
CSB-MP477896EOE
CSB-EP477896EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP477897EOE
CSB-EP477897EOE
CSB-BP477897EOE
CSB-MP477897EOE
CSB-EP477897EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0246 protein yaaA (yaaA) CSB-YP477898EOE
CSB-EP477898EOE
CSB-BP477898EOE
CSB-MP477898EOE
CSB-EP477898EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Crotonobetainyl-CoA:carnitine CoA-transferase (caiB) CSB-YP477899EOE
CSB-EP477899EOE
CSB-BP477899EOE
CSB-MP477899EOE
CSB-EP477899EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0761 membrane protein yihY (yihY), partial CSB-YP477900EOE1
CSB-EP477900EOE1
CSB-BP477900EOE1
CSB-MP477900EOE1
CSB-EP477900EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Cell division protein ZapB (zapB) CSB-YP477901EOE
CSB-EP477901EOE
CSB-BP477901EOE
CSB-MP477901EOE
CSB-EP477901EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP477902EOE
CSB-EP477902EOE
CSB-BP477902EOE
CSB-MP477902EOE
CSB-EP477902EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribulokinase (araB) CSB-YP477903EOE
CSB-EP477903EOE
CSB-BP477903EOE
CSB-MP477903EOE
CSB-EP477903EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP477904EOE
CSB-EP477904EOE
CSB-BP477904EOE
CSB-MP477904EOE
CSB-EP477904EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 GMP reductase (guaC) CSB-YP477905EOE
CSB-EP477905EOE
CSB-BP477905EOE
CSB-MP477905EOE
CSB-EP477905EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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