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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O157:H7 Putative N-acetylmannosamine-6-phosphate 2-epimerase (nanE) CSB-YP477866EOE
CSB-EP477866EOE
CSB-BP477866EOE
CSB-MP477866EOE
CSB-EP477866EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L13 (rplM) CSB-YP477867EOE
CSB-EP477867EOE
CSB-BP477867EOE
CSB-MP477867EOE
CSB-EP477867EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP477868EOE
CSB-EP477868EOE
CSB-BP477868EOE
CSB-MP477868EOE
CSB-EP477868EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Integration host factor subunit beta (ihfB) CSB-YP477869EOE
CSB-EP477869EOE
CSB-BP477869EOE
CSB-MP477869EOE
CSB-EP477869EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L18 (rplR) CSB-YP477870EOE
CSB-EP477870EOE
CSB-BP477870EOE
CSB-MP477870EOE
CSB-EP477870EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S17 (rpsQ) CSB-YP477871EOE
CSB-EP477871EOE
CSB-BP477871EOE
CSB-MP477871EOE
CSB-EP477871EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 30S ribosomal protein S10 (rpsJ) CSB-YP477872EOE
CSB-EP477872EOE
CSB-BP477872EOE
CSB-MP477872EOE
CSB-EP477872EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein slyX (slyX) CSB-YP477873EOE
CSB-EP477873EOE
CSB-BP477873EOE
CSB-MP477873EOE
CSB-EP477873EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 3-dehydroquinate synthase (aroB) CSB-YP477874EOE
CSB-EP477874EOE
CSB-BP477874EOE
CSB-MP477874EOE
CSB-EP477874EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Rhomboid protease glpG (glpG), partial CSB-YP477875EOE1
CSB-EP477875EOE1
CSB-BP477875EOE1
CSB-MP477875EOE1
CSB-EP477875EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable malonic semialdehyde reductase RutE (rutE) CSB-YP477876EOE
CSB-EP477876EOE
CSB-BP477876EOE
CSB-MP477876EOE
CSB-EP477876EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP477877EOE
CSB-EP477877EOE
CSB-BP477877EOE
CSB-MP477877EOE
CSB-EP477877EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Multidrug resistance protein MdtB (mdtB), partial CSB-YP477878EOE
CSB-EP477878EOE
CSB-BP477878EOE
CSB-MP477878EOE
CSB-EP477878EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Urease subunit beta (ureB) CSB-YP477879EOE
CSB-EP477879EOE
CSB-BP477879EOE
CSB-MP477879EOE
CSB-EP477879EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 D-tagatose-1,6-bisphosphate aldolase subunit GatY (gatY) CSB-YP477880EOE
CSB-EP477880EOE
CSB-BP477880EOE
CSB-MP477880EOE
CSB-EP477880EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0176 protein yceA (yceA) CSB-YP477881EOE
CSB-EP477881EOE
CSB-BP477881EOE
CSB-MP477881EOE
CSB-EP477881EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Flagellar P-ring protein (flgI) CSB-YP477882EOE
CSB-EP477882EOE
CSB-BP477882EOE
CSB-MP477882EOE
CSB-EP477882EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Beta-hexosaminidase (nagZ) CSB-YP477883EOE
CSB-EP477883EOE
CSB-BP477883EOE
CSB-MP477883EOE
CSB-EP477883EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP477884EOE
CSB-EP477884EOE
CSB-BP477884EOE
CSB-MP477884EOE
CSB-EP477884EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L28 (rpmB) CSB-YP477885EOE
CSB-EP477885EOE
CSB-BP477885EOE
CSB-MP477885EOE
CSB-EP477885EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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