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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida Ribosomal RNA small subunit methyltransferase C (rsmC) CSB-YP478086AZM
CSB-EP478086AZM
CSB-BP478086AZM
CSB-MP478086AZM
CSB-EP478086AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP478087AZM
CSB-EP478087AZM
CSB-BP478087AZM
CSB-MP478087AZM
CSB-EP478087AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Dihydroorotate dehydrogenase (quinone) CSB-YP478088AZM
CSB-EP478088AZM
CSB-BP478088AZM
CSB-MP478088AZM
CSB-EP478088AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Pantothenate synthetase (panC) CSB-YP478089AZM
CSB-EP478089AZM
CSB-BP478089AZM
CSB-MP478089AZM
CSB-EP478089AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Lipid II flippase FtsW (ftsW), partial CSB-YP478090AZM
CSB-EP478090AZM
CSB-BP478090AZM
CSB-MP478090AZM
CSB-EP478090AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Alanine racemase (alr) CSB-YP478091AZM
CSB-EP478091AZM
CSB-BP478091AZM
CSB-MP478091AZM
CSB-EP478091AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Bifunctional protein HldE (hldE) CSB-YP478092AZM
CSB-EP478092AZM
CSB-BP478092AZM
CSB-MP478092AZM
CSB-EP478092AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 3-dehydroquinate synthase (aroB) CSB-YP478093AZM
CSB-EP478093AZM
CSB-BP478093AZM
CSB-MP478093AZM
CSB-EP478093AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L31 (rpmE) CSB-YP478094AZM
CSB-EP478094AZM
CSB-BP478094AZM
CSB-MP478094AZM
CSB-EP478094AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida HTH-type transcriptional repressor NsrR (nsrR) CSB-YP478095AZM
CSB-EP478095AZM
CSB-BP478095AZM
CSB-MP478095AZM
CSB-EP478095AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Probable cytosol aminopeptidase (pepA), partial CSB-YP478096AZM
CSB-EP478096AZM
CSB-BP478096AZM
CSB-MP478096AZM
CSB-EP478096AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP478097AZM
CSB-EP478097AZM
CSB-BP478097AZM
CSB-MP478097AZM
CSB-EP478097AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP478098AZM
CSB-EP478098AZM
CSB-BP478098AZM
CSB-MP478098AZM
CSB-EP478098AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 3-dehydroquinate dehydratase (aroQ) CSB-YP478099AZM
CSB-EP478099AZM
CSB-BP478099AZM
CSB-MP478099AZM
CSB-EP478099AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Dihydrodipicolinate reductase (dapB) CSB-YP478100AZM
CSB-EP478100AZM
CSB-BP478100AZM
CSB-MP478100AZM
CSB-EP478100AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 30S ribosomal protein S15 (rpsO) CSB-YP478101AZM
CSB-EP478101AZM
CSB-BP478101AZM
CSB-MP478101AZM
CSB-EP478101AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Protein RecA (recA) CSB-YP478102AZM
CSB-EP478102AZM
CSB-BP478102AZM
CSB-MP478102AZM
CSB-EP478102AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L10 (rplJ) CSB-YP478103AZM
CSB-EP478103AZM
CSB-BP478103AZM
CSB-MP478103AZM
CSB-EP478103AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP478104AZM
CSB-EP478104AZM
CSB-BP478104AZM
CSB-MP478104AZM
CSB-EP478104AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida DNA replication and repair protein recF (recF) CSB-YP478105AZM
CSB-EP478105AZM
CSB-BP478105AZM
CSB-MP478105AZM
CSB-EP478105AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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