Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermodesulfovibrio yellowstonii NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP477846TMY
CSB-EP477846TMY
CSB-BP477846TMY
CSB-MP477846TMY
CSB-EP477846TMY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 tRNA 2-selenouridine synthase (selU) CSB-YP477847EOE
CSB-EP477847EOE
CSB-BP477847EOE
CSB-MP477847EOE
CSB-EP477847EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Carboxylate-amine ligase YbdK (ybdK) CSB-YP477848EOE
CSB-EP477848EOE
CSB-BP477848EOE
CSB-MP477848EOE
CSB-EP477848EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein CrcB homolog (crcB), partial CSB-YP477849EOE1
CSB-EP477849EOE1
CSB-BP477849EOE1
CSB-MP477849EOE1
CSB-EP477849EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L35 (rpmI) CSB-YP477850EOE
CSB-EP477850EOE
CSB-BP477850EOE
CSB-MP477850EOE
CSB-EP477850EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP477851EOE
CSB-EP477851EOE
CSB-BP477851EOE
CSB-MP477851EOE
CSB-EP477851EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Probable Fe (2+)-trafficking protein CSB-YP477852EOE
CSB-EP477852EOE
CSB-BP477852EOE
CSB-MP477852EOE
CSB-EP477852EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) CSB-YP477853EOE
CSB-EP477853EOE
CSB-BP477853EOE
CSB-MP477853EOE
CSB-EP477853EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glucosamine-6-phosphate deaminase (nagB) CSB-YP477854EOE
CSB-EP477854EOE
CSB-BP477854EOE
CSB-MP477854EOE
CSB-EP477854EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP477855EOE
CSB-EP477855EOE
CSB-BP477855EOE
CSB-MP477855EOE
CSB-EP477855EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0114 protein YqhA (yqhA), partial CSB-YP477856EOE1
CSB-EP477856EOE1
CSB-BP477856EOE1
CSB-MP477856EOE1
CSB-EP477856EOE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal RNA large subunit methyltransferase G (rlmG) CSB-YP477857EOE
CSB-EP477857EOE
CSB-BP477857EOE
CSB-MP477857EOE
CSB-EP477857EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP477858EOE
CSB-EP477858EOE
CSB-BP477858EOE
CSB-MP477858EOE
CSB-EP477858EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0597 protein yhaM (yhaM) CSB-YP477859EOE
CSB-EP477859EOE
CSB-BP477859EOE
CSB-MP477859EOE
CSB-EP477859EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 DnaA initiator-associating protein diaA (diaA) CSB-YP477860EOE
CSB-EP477860EOE
CSB-BP477860EOE
CSB-MP477860EOE
CSB-EP477860EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosome maturation factor RimP (rimP) CSB-YP477861EOE
CSB-EP477861EOE
CSB-BP477861EOE
CSB-MP477861EOE
CSB-EP477861EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 50S ribosomal protein L27 (rpmA) CSB-YP477862EOE
CSB-EP477862EOE
CSB-BP477862EOE
CSB-MP477862EOE
CSB-EP477862EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 UPF0194 membrane protein ybhG (ybhG) CSB-YP477863EOE
CSB-EP477863EOE
CSB-BP477863EOE
CSB-MP477863EOE
CSB-EP477863EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Ribosomal protein S6 modification protein (rimK) CSB-YP477864EOE
CSB-EP477864EOE
CSB-BP477864EOE
CSB-MP477864EOE
CSB-EP477864EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Protein mtfA (mtfA) CSB-YP477865EOE
CSB-EP477865EOE
CSB-BP477865EOE
CSB-MP477865EOE
CSB-EP477865EOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>