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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund L-rhamnose-proton symporter (rhaT), partial CSB-YP480239SWV
CSB-EP480239SWV
CSB-BP480239SWV
CSB-MP480239SWV
CSB-EP480239SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Regulator of ribonuclease activity A (rraA) CSB-YP480240SWV
CSB-EP480240SWV
CSB-BP480240SWV
CSB-MP480240SWV
CSB-EP480240SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable rRNA maturation factor YbeY (ybeY) CSB-YP480241SWV
CSB-EP480241SWV
CSB-BP480241SWV
CSB-MP480241SWV
CSB-EP480241SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Endonuclease 8 (nei) CSB-YP480242SWV
CSB-EP480242SWV
CSB-BP480242SWV
CSB-MP480242SWV
CSB-EP480242SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Chorismate synthase (aroC) CSB-YP480243SWV
CSB-EP480243SWV
CSB-BP480243SWV
CSB-MP480243SWV
CSB-EP480243SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Putative ion-transport protein yfeO (yfeO), partial CSB-YP480244SWV
CSB-EP480244SWV
CSB-BP480244SWV
CSB-MP480244SWV
CSB-EP480244SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Acetylornithine deacetylase (argE) CSB-YP480245SWV
CSB-EP480245SWV
CSB-BP480245SWV
CSB-MP480245SWV
CSB-EP480245SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L10 (rplJ) CSB-YP480246SWV
CSB-EP480246SWV
CSB-BP480246SWV
CSB-MP480246SWV
CSB-EP480246SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Regulator of sigma D (rsd) CSB-YP480247SWV
CSB-EP480247SWV
CSB-BP480247SWV
CSB-MP480247SWV
CSB-EP480247SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Homoserine O-succinyltransferase (metA) CSB-YP480248SWV
CSB-EP480248SWV
CSB-BP480248SWV
CSB-MP480248SWV
CSB-EP480248SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 4-hydroxybenzoate octaprenyltransferase (ubiA), partial CSB-YP480249SWV1
CSB-EP480249SWV1
CSB-BP480249SWV1
CSB-MP480249SWV1
CSB-EP480249SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Biotin synthase (bioB) CSB-YP480250SWV
CSB-EP480250SWV
CSB-BP480250SWV
CSB-MP480250SWV
CSB-EP480250SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP480251SWV
CSB-EP480251SWV
CSB-BP480251SWV
CSB-MP480251SWV
CSB-EP480251SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP480252SWV
CSB-EP480252SWV
CSB-BP480252SWV
CSB-MP480252SWV
CSB-EP480252SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cytoskeleton protein rodZ (rodZ), partial CSB-YP480253SWV
CSB-EP480253SWV
CSB-BP480253SWV
CSB-MP480253SWV
CSB-EP480253SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosomal protein S6 modification protein (rimK) CSB-YP480254SWV
CSB-EP480254SWV
CSB-BP480254SWV
CSB-MP480254SWV
CSB-EP480254SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Uncharacterized MFS-type transporter ycaD (ycaD), partial CSB-YP480255SWV
CSB-EP480255SWV
CSB-BP480255SWV
CSB-MP480255SWV
CSB-EP480255SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Integration host factor subunit beta (ihfB) CSB-YP480256SWV
CSB-EP480256SWV
CSB-BP480256SWV
CSB-MP480256SWV
CSB-EP480256SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L19 (rplS) CSB-YP480257SWV
CSB-EP480257SWV
CSB-BP480257SWV
CSB-MP480257SWV
CSB-EP480257SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 10 kDa chaperonin (groS) CSB-YP480258SWV
CSB-EP480258SWV
CSB-BP480258SWV
CSB-MP480258SWV
CSB-EP480258SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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