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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli UPF0234 protein yajQ (yajQ) CSB-YP481440ENW
CSB-EP481440ENW
CSB-BP481440ENW
CSB-MP481440ENW
CSB-EP481440ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetaldehyde dehydrogenase (mhpF) CSB-YP481441ENW
CSB-EP481441ENW
CSB-BP481441ENW
CSB-MP481441ENW
CSB-EP481441ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Gamma-glutamyl phosphate reductase (proA) CSB-YP481442ENW
CSB-EP481442ENW
CSB-BP481442ENW
CSB-MP481442ENW
CSB-EP481442ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L31 type B (rpmE2) CSB-YP481443ENW
CSB-EP481443ENW
CSB-BP481443ENW
CSB-MP481443ENW
CSB-EP481443ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Carboxylate-amine ligase YbdK (ybdK) CSB-YP481444ENW
CSB-EP481444ENW
CSB-BP481444ENW
CSB-MP481444ENW
CSB-EP481444ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar transcriptional regulator FlhD (flhD) CSB-YP481445ENW
CSB-EP481445ENW
CSB-BP481445ENW
CSB-MP481445ENW
CSB-EP481445ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Molecular chaperone Hsp31 and glyoxalase 3 (hchA) CSB-YP481446ENW
CSB-EP481446ENW
CSB-BP481446ENW
CSB-MP481446ENW
CSB-EP481446ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0250 protein ybeD (ybeD) CSB-YP481447ENW
CSB-EP481447ENW
CSB-BP481447ENW
CSB-MP481447ENW
CSB-EP481447ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L25 (rplY) CSB-YP481448ENW
CSB-EP481448ENW
CSB-BP481448ENW
CSB-MP481448ENW
CSB-EP481448ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable malate:quinone oxidoreductase (mqo) CSB-YP481449ENW
CSB-EP481449ENW
CSB-BP481449ENW
CSB-MP481449ENW
CSB-EP481449ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-tagatose-1,6-bisphosphate aldolase subunit KbaY (kbaY) CSB-YP481450ENW
CSB-EP481450ENW
CSB-BP481450ENW
CSB-MP481450ENW
CSB-EP481450ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosome maturation factor RimP (rimP) CSB-YP481451ENW
CSB-EP481451ENW
CSB-BP481451ENW
CSB-MP481451ENW
CSB-EP481451ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP481452ENW
CSB-EP481452ENW
CSB-BP481452ENW
CSB-MP481452ENW
CSB-EP481452ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S9 (rpsI) CSB-YP481453ENW
CSB-EP481453ENW
CSB-BP481453ENW
CSB-MP481453ENW
CSB-EP481453ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Shikimate dehydrogenase (aroE) CSB-YP481454ENW
CSB-EP481454ENW
CSB-BP481454ENW
CSB-MP481454ENW
CSB-EP481454ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S11 (rpsK) CSB-YP481455ENW
CSB-EP481455ENW
CSB-BP481455ENW
CSB-MP481455ENW
CSB-EP481455ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L5 (rplE) CSB-YP481456ENW
CSB-EP481456ENW
CSB-BP481456ENW
CSB-MP481456ENW
CSB-EP481456ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S3 (rpsC) CSB-YP481457ENW
CSB-EP481457ENW
CSB-BP481457ENW
CSB-MP481457ENW
CSB-EP481457ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S10 (rpsJ) CSB-YP481458ENW
CSB-EP481458ENW
CSB-BP481458ENW
CSB-MP481458ENW
CSB-EP481458ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein slyX (slyX) CSB-YP481459ENW
CSB-EP481459ENW
CSB-BP481459ENW
CSB-MP481459ENW
CSB-EP481459ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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