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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella schwarzengrund tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP480279SWV
CSB-EP480279SWV
CSB-BP480279SWV
CSB-MP480279SWV
CSB-EP480279SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0306 protein yhbP (yhbP) CSB-YP480280SWV
CSB-EP480280SWV
CSB-BP480280SWV
CSB-MP480280SWV
CSB-EP480280SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L21 (rplU) CSB-YP480281SWV
CSB-EP480281SWV
CSB-BP480281SWV
CSB-MP480281SWV
CSB-EP480281SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S9 (rpsI) CSB-YP480282SWV
CSB-EP480282SWV
CSB-BP480282SWV
CSB-MP480282SWV
CSB-EP480282SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP480283SWV
CSB-EP480283SWV
CSB-BP480283SWV
CSB-MP480283SWV
CSB-EP480283SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Probable crotonobetaine/carnitine-CoA ligase (caiC), partial CSB-YP480284SWV
CSB-EP480284SWV
CSB-BP480284SWV
CSB-MP480284SWV
CSB-EP480284SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP480285SWV
CSB-EP480285SWV
CSB-BP480285SWV
CSB-MP480285SWV
CSB-EP480285SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0283 membrane protein ycjF (ycjF), partial CSB-YP480286SWV1
CSB-EP480286SWV1
CSB-BP480286SWV1
CSB-MP480286SWV1
CSB-EP480286SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S11 (rpsK) CSB-YP480287SWV
CSB-EP480287SWV
CSB-BP480287SWV
CSB-MP480287SWV
CSB-EP480287SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 50S ribosomal protein L24 (rplX) CSB-YP480288SWV
CSB-EP480288SWV
CSB-BP480288SWV
CSB-MP480288SWV
CSB-EP480288SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S19 (rpsS) CSB-YP480289SWV
CSB-EP480289SWV
CSB-BP480289SWV
CSB-MP480289SWV
CSB-EP480289SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 30S ribosomal protein S7 (rpsG) CSB-YP480290SWV
CSB-EP480290SWV
CSB-BP480290SWV
CSB-MP480290SWV
CSB-EP480290SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP480291SWV
CSB-EP480291SWV
CSB-BP480291SWV
CSB-MP480291SWV
CSB-EP480291SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Aspartate 1-decarboxylase (panD) CSB-YP480292SWV
CSB-EP480292SWV
CSB-BP480292SWV
CSB-MP480292SWV
CSB-EP480292SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0325 protein yaeH (yaeH) CSB-YP480293SWV
CSB-EP480293SWV
CSB-BP480293SWV
CSB-MP480293SWV
CSB-EP480293SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP480294SWV
CSB-EP480294SWV
CSB-BP480294SWV
CSB-MP480294SWV
CSB-EP480294SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund UPF0745 protein YcgL (ycgL) CSB-YP480295SWV
CSB-EP480295SWV
CSB-BP480295SWV
CSB-MP480295SWV
CSB-EP480295SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Protein mgrB (mgrB), partial CSB-YP480296SWV
CSB-EP480296SWV
CSB-BP480296SWV
CSB-MP480296SWV
CSB-EP480296SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Ferrous iron transport protein C (feoC) CSB-YP480297SWV
CSB-EP480297SWV
CSB-BP480297SWV
CSB-MP480297SWV
CSB-EP480297SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP480298SWV
CSB-EP480298SWV
CSB-BP480298SWV
CSB-MP480298SWV
CSB-EP480298SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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