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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi A UPF0263 protein yciU (yciU) CSB-YP480439SWT
CSB-EP480439SWT
CSB-BP480439SWT
CSB-MP480439SWT
CSB-EP480439SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Tryptophan synthase beta chain (trpB) CSB-YP480440SWT
CSB-EP480440SWT
CSB-BP480440SWT
CSB-MP480440SWT
CSB-EP480440SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Multidrug resistance protein MdtL (mdtL), partial CSB-YP480441SWT
CSB-EP480441SWT
CSB-BP480441SWT
CSB-MP480441SWT
CSB-EP480441SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Dihydroxy-acid dehydratase (ilvD), partial CSB-YP480442SWT
CSB-EP480442SWT
CSB-BP480442SWT
CSB-MP480442SWT
CSB-EP480442SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP480443SWT
CSB-EP480443SWT
CSB-BP480443SWT
CSB-MP480443SWT
CSB-EP480443SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP480444SWT
CSB-EP480444SWT
CSB-BP480444SWT
CSB-MP480444SWT
CSB-EP480444SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Rhamnulose-1-phosphate aldolase (rhaD) CSB-YP480445SWT
CSB-EP480445SWT
CSB-BP480445SWT
CSB-MP480445SWT
CSB-EP480445SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Catalase-peroxidase (katG), partial CSB-YP480446SWT
CSB-EP480446SWT
CSB-BP480446SWT
CSB-MP480446SWT
CSB-EP480446SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 50S ribosomal protein L1 (rplA) CSB-YP480447SWT
CSB-EP480447SWT
CSB-BP480447SWT
CSB-MP480447SWT
CSB-EP480447SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A NADH pyrophosphatase (nudC) CSB-YP480448SWT
CSB-EP480448SWT
CSB-BP480448SWT
CSB-MP480448SWT
CSB-EP480448SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Peptidase E (pepE) CSB-YP480449SWT
CSB-EP480449SWT
CSB-BP480449SWT
CSB-MP480449SWT
CSB-EP480449SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP480450SWT
CSB-EP480450SWT
CSB-BP480450SWT
CSB-MP480450SWT
CSB-EP480450SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A DNA replication terminus site-binding protein (tus) CSB-YP480451SWT
CSB-EP480451SWT
CSB-BP480451SWT
CSB-MP480451SWT
CSB-EP480451SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP480452SWT
CSB-EP480452SWT
CSB-BP480452SWT
CSB-MP480452SWT
CSB-EP480452SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Elongation factor P (efp) CSB-YP480453SWT
CSB-EP480453SWT
CSB-BP480453SWT
CSB-MP480453SWT
CSB-EP480453SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Adenylosuccinate synthetase (purA) CSB-YP480454SWT
CSB-EP480454SWT
CSB-BP480454SWT
CSB-MP480454SWT
CSB-EP480454SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A L-ribulose-5-phosphate 3-epimerase UlaE (ulaE) CSB-YP480455SWT
CSB-EP480455SWT
CSB-BP480455SWT
CSB-MP480455SWT
CSB-EP480455SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP480456SWT
CSB-EP480456SWT
CSB-BP480456SWT
CSB-MP480456SWT
CSB-EP480456SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0386 protein yjhX (yjhX) CSB-YP480457SWT
CSB-EP480457SWT
CSB-BP480457SWT
CSB-MP480457SWT
CSB-EP480457SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Protein ApaG (apaG) CSB-YP480458SWT
CSB-EP480458SWT
CSB-BP480458SWT
CSB-MP480458SWT
CSB-EP480458SWT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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