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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lycosa singoriensis U15-lycotoxin-Ls1d CSB-YP481360LRO
CSB-EP481360LRO
CSB-BP481360LRO
CSB-MP481360LRO
CSB-EP481360LRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lycosa singoriensis U19-lycotoxin-Ls1a CSB-YP481361LRO
CSB-EP481361LRO
CSB-BP481361LRO
CSB-MP481361LRO
CSB-EP481361LRO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leymus chinensis Fructan 1-exohydrolase (1-FEH), partial CSB-YP481362LPJ
CSB-EP481362LPJ
CSB-BP481362LPJ
CSB-MP481362LPJ
CSB-EP481362LPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida tRNA (guanine-N (1)-)-methyltransferase CSB-YP481363AZM
CSB-EP481363AZM
CSB-BP481363AZM
CSB-MP481363AZM
CSB-EP481363AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Trp operon repressor homolog (trpR) CSB-YP481364AZM
CSB-EP481364AZM
CSB-BP481364AZM
CSB-MP481364AZM
CSB-EP481364AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Electron transport complex protein RnfD (rnfD), partial CSB-YP481365AZM1
CSB-EP481365AZM1
CSB-BP481365AZM1
CSB-MP481365AZM1
CSB-EP481365AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida tRNA (cmo5U34)-methyltransferase CSB-YP481366AZM
CSB-EP481366AZM
CSB-BP481366AZM
CSB-MP481366AZM
CSB-EP481366AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida L-alanine exporter AlaE (alaE), partial CSB-YP481367AZM1
CSB-EP481367AZM1
CSB-BP481367AZM1
CSB-MP481367AZM1
CSB-EP481367AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP481368AZM
CSB-EP481368AZM
CSB-BP481368AZM
CSB-MP481368AZM
CSB-EP481368AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phosphomethylpyrimidine synthase (thiC), partial CSB-YP481369AZM
CSB-EP481369AZM
CSB-BP481369AZM
CSB-MP481369AZM
CSB-EP481369AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP481370AZM
CSB-EP481370AZM
CSB-BP481370AZM
CSB-MP481370AZM
CSB-EP481370AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Cysteine--tRNA ligase (cysS) CSB-YP481371AZM
CSB-EP481371AZM
CSB-BP481371AZM
CSB-MP481371AZM
CSB-EP481371AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Probable ubiquinone biosynthesis protein UbiB (ubiB), partial CSB-YP481372AZM1
CSB-EP481372AZM1
CSB-BP481372AZM1
CSB-MP481372AZM1
CSB-EP481372AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Glutamyl-tRNA reductase (hemA) CSB-YP481373AZM
CSB-EP481373AZM
CSB-BP481373AZM
CSB-MP481373AZM
CSB-EP481373AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Trigger factor (tig) CSB-YP481374AZM
CSB-EP481374AZM
CSB-BP481374AZM
CSB-MP481374AZM
CSB-EP481374AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Putative reductase VSAL_I2028 (VSAL_I2028) CSB-YP481375AZM
CSB-EP481375AZM
CSB-BP481375AZM
CSB-MP481375AZM
CSB-EP481375AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida ATP synthase subunit c (atpE), partial CSB-YP481376AZM1
CSB-EP481376AZM1
CSB-BP481376AZM1
CSB-MP481376AZM1
CSB-EP481376AZM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L25 (rplY) CSB-YP481377AZM
CSB-EP481377AZM
CSB-BP481377AZM
CSB-MP481377AZM
CSB-EP481377AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Transcriptional repressor NrdR (nrdR) CSB-YP481378AZM
CSB-EP481378AZM
CSB-BP481378AZM
CSB-MP481378AZM
CSB-EP481378AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Xanthine phosphoribosyltransferase (gpt) CSB-YP481379AZM
CSB-EP481379AZM
CSB-BP481379AZM
CSB-MP481379AZM
CSB-EP481379AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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