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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rhizobium leguminosarum bv. trifolii Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP481299RKQ
CSB-EP481299RKQ
CSB-BP481299RKQ
CSB-MP481299RKQ
CSB-EP481299RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Glutamate--tRNA ligase (gltX) CSB-YP481300RKQ
CSB-EP481300RKQ
CSB-BP481300RKQ
CSB-MP481300RKQ
CSB-EP481300RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Histidine--tRNA ligase (hisS), partial CSB-YP481301RKQ
CSB-EP481301RKQ
CSB-BP481301RKQ
CSB-MP481301RKQ
CSB-EP481301RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0283 membrane protein Rleg2_1967 (Rleg2_1967), partial CSB-YP481302RKQ1
CSB-EP481302RKQ1
CSB-BP481302RKQ1
CSB-MP481302RKQ1
CSB-EP481302RKQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii DNA mismatch repair protein mutL (mutL), partial CSB-YP481303RKQ
CSB-EP481303RKQ
CSB-BP481303RKQ
CSB-MP481303RKQ
CSB-EP481303RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP481305RKQ
CSB-EP481305RKQ
CSB-BP481305RKQ
CSB-MP481305RKQ
CSB-EP481305RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 3-dehydroquinate synthase (aroB) CSB-YP481306RKQ
CSB-EP481306RKQ
CSB-BP481306RKQ
CSB-MP481306RKQ
CSB-EP481306RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii UPF0301 protein Rleg2_0617 (Rleg2_0617) CSB-YP481307RKQ
CSB-EP481307RKQ
CSB-BP481307RKQ
CSB-MP481307RKQ
CSB-EP481307RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP481308RKQ
CSB-EP481308RKQ
CSB-BP481308RKQ
CSB-MP481308RKQ
CSB-EP481308RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribosome maturation factor RimM (rimM) CSB-YP481309RKQ
CSB-EP481309RKQ
CSB-BP481309RKQ
CSB-MP481309RKQ
CSB-EP481309RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Monofunctional biosynthetic peptidoglycan transglycosylase (mtgA), partial CSB-YP481310RKQ1
CSB-EP481310RKQ1
CSB-BP481310RKQ1
CSB-MP481310RKQ1
CSB-EP481310RKQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP481311RKQ
CSB-EP481311RKQ
CSB-BP481311RKQ
CSB-MP481311RKQ
CSB-EP481311RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP481312RKQ
CSB-EP481312RKQ
CSB-BP481312RKQ
CSB-MP481312RKQ
CSB-EP481312RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP481313RKQ
CSB-EP481313RKQ
CSB-BP481313RKQ
CSB-MP481313RKQ
CSB-EP481313RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP481314RKQ
CSB-EP481314RKQ
CSB-BP481314RKQ
CSB-MP481314RKQ
CSB-EP481314RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 30S ribosomal protein S6 (rpsF) CSB-YP481315RKQ
CSB-EP481315RKQ
CSB-BP481315RKQ
CSB-MP481315RKQ
CSB-EP481315RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP481316RKQ
CSB-EP481316RKQ
CSB-BP481316RKQ
CSB-MP481316RKQ
CSB-EP481316RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP481317RKQ
CSB-EP481317RKQ
CSB-BP481317RKQ
CSB-MP481317RKQ
CSB-EP481317RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Aspartate--tRNA ligase (aspS), partial CSB-YP481318RKQ
CSB-EP481318RKQ
CSB-BP481318RKQ
CSB-MP481318RKQ
CSB-EP481318RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Uracil phosphoribosyltransferase (upp) CSB-YP481319RKQ
CSB-EP481319RKQ
CSB-BP481319RKQ
CSB-MP481319RKQ
CSB-EP481319RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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