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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aliivibrio salmonicida Anaerobic nitric oxide reductase flavorubredoxin (norV) CSB-YP481380AZM
CSB-EP481380AZM
CSB-BP481380AZM
CSB-MP481380AZM
CSB-EP481380AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Probable septum site-determining protein MinC (minC) CSB-YP481381AZM
CSB-EP481381AZM
CSB-BP481381AZM
CSB-MP481381AZM
CSB-EP481381AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Methionine--tRNA ligase (metG), partial CSB-YP481382AZM
CSB-EP481382AZM
CSB-BP481382AZM
CSB-MP481382AZM
CSB-EP481382AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Recombination protein RecR (recR) CSB-YP481383AZM
CSB-EP481383AZM
CSB-BP481383AZM
CSB-MP481383AZM
CSB-EP481383AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Tryptophan synthase alpha chain (trpA) CSB-YP481384AZM
CSB-EP481384AZM
CSB-BP481384AZM
CSB-MP481384AZM
CSB-EP481384AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Dihydrodipicolinate synthase (dapA) CSB-YP481385AZM
CSB-EP481385AZM
CSB-BP481385AZM
CSB-MP481385AZM
CSB-EP481385AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP481386AZM
CSB-EP481386AZM
CSB-BP481386AZM
CSB-MP481386AZM
CSB-EP481386AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Protein RnfH (rnfH) CSB-YP481387AZM
CSB-EP481387AZM
CSB-BP481387AZM
CSB-MP481387AZM
CSB-EP481387AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida tRNA pseudouridine synthase D (truD) CSB-YP481388AZM
CSB-EP481388AZM
CSB-BP481388AZM
CSB-MP481388AZM
CSB-EP481388AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP481389AZM
CSB-EP481389AZM
CSB-BP481389AZM
CSB-MP481389AZM
CSB-EP481389AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (mtnN) CSB-YP481390AZM
CSB-EP481390AZM
CSB-BP481390AZM
CSB-MP481390AZM
CSB-EP481390AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L27 (rpmA) CSB-YP481391AZM
CSB-EP481391AZM
CSB-BP481391AZM
CSB-MP481391AZM
CSB-EP481391AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP481392AZM
CSB-EP481392AZM
CSB-BP481392AZM
CSB-MP481392AZM
CSB-EP481392AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP481393AZM
CSB-EP481393AZM
CSB-BP481393AZM
CSB-MP481393AZM
CSB-EP481393AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Phosphoadenosine phosphosulfate reductase (cysH) CSB-YP481394AZM
CSB-EP481394AZM
CSB-BP481394AZM
CSB-MP481394AZM
CSB-EP481394AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Multifunctional CCA protein (cca) CSB-YP481395AZM
CSB-EP481395AZM
CSB-BP481395AZM
CSB-MP481395AZM
CSB-EP481395AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida UPF0042 nucleotide-binding protein VSAL_I0495 (VSAL_I0495) CSB-YP481396AZM
CSB-EP481396AZM
CSB-BP481396AZM
CSB-MP481396AZM
CSB-EP481396AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida N-succinylglutamate 5-semialdehyde dehydrogenase (astD) CSB-YP481397AZM
CSB-EP481397AZM
CSB-BP481397AZM
CSB-MP481397AZM
CSB-EP481397AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida 50S ribosomal protein L9 (rplI) CSB-YP481398AZM
CSB-EP481398AZM
CSB-BP481398AZM
CSB-MP481398AZM
CSB-EP481398AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Arginine deiminase (arcA) CSB-YP481399AZM
CSB-EP481399AZM
CSB-BP481399AZM
CSB-MP481399AZM
CSB-EP481399AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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