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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Coxiella burnetii GTPase Der (der) CSB-YP481600DXN
CSB-EP481600DXN
CSB-BP481600DXN
CSB-MP481600DXN
CSB-EP481600DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Chaperone protein DnaJ (dnaJ) CSB-YP481601DXN
CSB-EP481601DXN
CSB-BP481601DXN
CSB-MP481601DXN
CSB-EP481601DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 50S ribosomal protein L20 (rplT) CSB-YP481602DXN
CSB-EP481602DXN
CSB-BP481602DXN
CSB-MP481602DXN
CSB-EP481602DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP481603DXN
CSB-EP481603DXN
CSB-BP481603DXN
CSB-MP481603DXN
CSB-EP481603DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP481604DXN
CSB-EP481604DXN
CSB-BP481604DXN
CSB-MP481604DXN
CSB-EP481604DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii tRNA (guanine-N (1)-)-methyltransferase CSB-YP481605DXN
CSB-EP481605DXN
CSB-BP481605DXN
CSB-MP481605DXN
CSB-EP481605DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii DNA replication and repair protein recF (recF) CSB-YP481606DXN
CSB-EP481606DXN
CSB-BP481606DXN
CSB-MP481606DXN
CSB-EP481606DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP481607DXN
CSB-EP481607DXN
CSB-BP481607DXN
CSB-MP481607DXN
CSB-EP481607DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP481608DXN
CSB-EP481608DXN
CSB-BP481608DXN
CSB-MP481608DXN
CSB-EP481608DXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP481609OED
CSB-EP481609OED
CSB-BP481609OED
CSB-MP481609OED
CSB-EP481609OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans ATP-dependent protease subunit HslV (hslV) CSB-YP481610OED
CSB-EP481610OED
CSB-BP481610OED
CSB-MP481610OED
CSB-EP481610OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Bifunctional protein FolD (folD) CSB-YP481611OED
CSB-EP481611OED
CSB-BP481611OED
CSB-MP481611OED
CSB-EP481611OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 2-phospho-L-lactate guanylyltransferase (cofC) CSB-YP481612OED
CSB-EP481612OED
CSB-BP481612OED
CSB-MP481612OED
CSB-EP481612OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L35 (rpmI) CSB-YP481613OED
CSB-EP481613OED
CSB-BP481613OED
CSB-MP481613OED
CSB-EP481613OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S20 (rpsT) CSB-YP481614OED
CSB-EP481614OED
CSB-BP481614OED
CSB-MP481614OED
CSB-EP481614OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP481615OED
CSB-EP481615OED
CSB-BP481615OED
CSB-MP481615OED
CSB-EP481615OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L21 (rplU) CSB-YP481616OED
CSB-EP481616OED
CSB-BP481616OED
CSB-MP481616OED
CSB-EP481616OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans Biotin synthase (bioB) CSB-YP481617OED
CSB-EP481617OED
CSB-BP481617OED
CSB-MP481617OED
CSB-EP481617OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 30S ribosomal protein S7 (rpsG) CSB-YP481618OED
CSB-EP481618OED
CSB-BP481618OED
CSB-MP481618OED
CSB-EP481618OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligotropha carboxidovorans 50S ribosomal protein L2 (rplB) CSB-YP481619OED
CSB-EP481619OED
CSB-BP481619OED
CSB-MP481619OED
CSB-EP481619OED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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