Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli O45:K1 Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP482601EOK
CSB-EP482601EOK
CSB-BP482601EOK
CSB-MP482601EOK
CSB-EP482601EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP482602EOK
CSB-EP482602EOK
CSB-BP482602EOK
CSB-MP482602EOK
CSB-EP482602EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP482603EOK
CSB-EP482603EOK
CSB-BP482603EOK
CSB-MP482603EOK
CSB-EP482603EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP482604EOK
CSB-EP482604EOK
CSB-BP482604EOK
CSB-MP482604EOK
CSB-EP482604EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 DNA gyrase inhibitor YacG (yacG) CSB-YP482605EOK
CSB-EP482605EOK
CSB-BP482605EOK
CSB-MP482605EOK
CSB-EP482605EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP482606EOK
CSB-EP482606EOK
CSB-BP482606EOK
CSB-MP482606EOK
CSB-EP482606EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 N-succinylarginine dihydrolase (astB) CSB-YP482607EOK
CSB-EP482607EOK
CSB-BP482607EOK
CSB-MP482607EOK
CSB-EP482607EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 G/U mismatch-specific DNA glycosylase (mug) CSB-YP482608EOK
CSB-EP482608EOK
CSB-BP482608EOK
CSB-MP482608EOK
CSB-EP482608EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UPF0102 protein yraN (yraN) CSB-YP482609EOK
CSB-EP482609EOK
CSB-BP482609EOK
CSB-MP482609EOK
CSB-EP482609EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 30S ribosomal protein S15 (rpsO) CSB-YP482610EOK
CSB-EP482610EOK
CSB-BP482610EOK
CSB-MP482610EOK
CSB-EP482610EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UPF0231 protein yacL (yacL) CSB-YP482611EOK
CSB-EP482611EOK
CSB-BP482611EOK
CSB-MP482611EOK
CSB-EP482611EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP482612EOK
CSB-EP482612EOK
CSB-BP482612EOK
CSB-MP482612EOK
CSB-EP482612EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 [Protein-PII] uridylyltransferase (glnD), partial CSB-YP482613EOK
CSB-EP482613EOK
CSB-BP482613EOK
CSB-MP482613EOK
CSB-EP482613EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP482614EOK
CSB-EP482614EOK
CSB-BP482614EOK
CSB-MP482614EOK
CSB-EP482614EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UPF0253 protein yaeP (yaeP) CSB-YP482615EOK
CSB-EP482615EOK
CSB-BP482615EOK
CSB-MP482615EOK
CSB-EP482615EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Uncharacterized Nudix hydrolase NudL (nudL) CSB-YP482616EOK
CSB-EP482616EOK
CSB-BP482616EOK
CSB-MP482616EOK
CSB-EP482616EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP482617EOK
CSB-EP482617EOK
CSB-BP482617EOK
CSB-MP482617EOK
CSB-EP482617EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 tRNA (mo5U34)-methyltransferase CSB-YP482618EOK
CSB-EP482618EOK
CSB-BP482618EOK
CSB-MP482618EOK
CSB-EP482618EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UPF0042 nucleotide-binding protein yhbJ (yhbJ) CSB-YP482619EOK
CSB-EP482619EOK
CSB-BP482619EOK
CSB-MP482619EOK
CSB-EP482619EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 50S ribosomal protein L13 (rplM) CSB-YP482620EOK
CSB-EP482620EOK
CSB-BP482620EOK
CSB-MP482620EOK
CSB-EP482620EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>