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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP481520ENW
CSB-EP481520ENW
CSB-BP481520ENW
CSB-MP481520ENW
CSB-EP481520ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0225 protein ychJ (ychJ) CSB-YP481521ENW
CSB-EP481521ENW
CSB-BP481521ENW
CSB-MP481521ENW
CSB-EP481521ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP481522ENW
CSB-EP481522ENW
CSB-BP481522ENW
CSB-MP481522ENW
CSB-EP481522ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Zinc transport protein ZntB (zntB), partial CSB-YP481523ENW1
CSB-EP481523ENW1
CSB-BP481523ENW1
CSB-MP481523ENW1
CSB-EP481523ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Trans-aconitate 2-methyltransferase (tam) CSB-YP481524ENW
CSB-EP481524ENW
CSB-BP481524ENW
CSB-MP481524ENW
CSB-EP481524ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Spermidine export protein MdtJ (mdtJ), partial CSB-YP481525ENW1
CSB-EP481525ENW1
CSB-BP481525ENW1
CSB-MP481525ENW1
CSB-EP481525ENW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Electron transport complex protein RnfD (rnfD), partial CSB-YP481526ENW
CSB-EP481526ENW
CSB-BP481526ENW
CSB-MP481526ENW
CSB-EP481526ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Multidrug resistance protein MdtK (mdtK), partial CSB-YP481527ENW
CSB-EP481527ENW
CSB-BP481527ENW
CSB-MP481527ENW
CSB-EP481527ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 50S ribosomal protein L35 (rpmI) CSB-YP481528ENW
CSB-EP481528ENW
CSB-BP481528ENW
CSB-MP481528ENW
CSB-EP481528ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-succinylarginine dihydrolase (astB) CSB-YP481529ENW
CSB-EP481529ENW
CSB-BP481529ENW
CSB-MP481529ENW
CSB-EP481529ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0181 protein yoaH (yoaH) CSB-YP481530ENW
CSB-EP481530ENW
CSB-BP481530ENW
CSB-MP481530ENW
CSB-EP481530ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal RNA small subunit methyltransferase F (rsmF) CSB-YP481531ENW
CSB-EP481531ENW
CSB-BP481531ENW
CSB-MP481531ENW
CSB-EP481531ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 50S ribosomal protein L13 (rplM) CSB-YP481532RLY
CSB-EP481532RLY
CSB-BP481532RLY
CSB-MP481532RLY
CSB-EP481532RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Transcriptional repressor NrdR (nrdR) CSB-YP481533RLY
CSB-EP481533RLY
CSB-BP481533RLY
CSB-MP481533RLY
CSB-EP481533RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Glutamyl-tRNA (Gln) amidotransferase subunit A, partial CSB-YP481534RLY
CSB-EP481534RLY
CSB-BP481534RLY
CSB-MP481534RLY
CSB-EP481534RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Deoxycytidine triphosphate deaminase (dcd) CSB-YP481535RLY
CSB-EP481535RLY
CSB-BP481535RLY
CSB-MP481535RLY
CSB-EP481535RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum 50S ribosomal protein L34 (rpmH) CSB-YP481536RLY
CSB-EP481536RLY
CSB-BP481536RLY
CSB-MP481536RLY
CSB-EP481536RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum UPF0133 protein RC1_2305 (RC1_2305) CSB-YP481537RLY
CSB-EP481537RLY
CSB-BP481537RLY
CSB-MP481537RLY
CSB-EP481537RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum ATP synthase subunit b 1 (atpF1), partial CSB-YP481538RLY
CSB-EP481538RLY
CSB-BP481538RLY
CSB-MP481538RLY
CSB-EP481538RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum DNA ligase (ligA), partial CSB-YP481539RLY
CSB-EP481539RLY
CSB-BP481539RLY
CSB-MP481539RLY
CSB-EP481539RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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