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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bovine Protein FAM149B1 (FAM149B1) CSB-YP008083BO
CSB-EP008083BO
CSB-BP008083BO
CSB-MP008083BO
CSB-EP008083BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Calpain-7 (CAPN7), partial CSB-YP004500PI
CSB-EP004500PI
CSB-BP004500PI
CSB-MP004500PI
CSB-EP004500PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Kinesin-like protein KIF3C (KIF3C), partial CSB-YP012337BO
CSB-EP012337BO
CSB-BP012337BO
CSB-MP012337BO
CSB-EP012337BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine DNA-directed RNA polymerase III subunit RPC9 (CRCP) CSB-YP005945BO
CSB-EP005945BO
CSB-BP005945BO
CSB-MP005945BO
CSB-EP005945BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Centrosomal protein of 120 kDa (CEP120), partial CSB-YP005232BO
CSB-EP005232BO
CSB-BP005232BO
CSB-MP005232BO
CSB-EP005232BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine SAM and SH3 domain-containing protein 3 (SASH3) CSB-YP020715BO
CSB-EP020715BO
CSB-BP020715BO
CSB-MP020715BO
CSB-EP020715BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine DIS3-like exonuclease 1 (DIS3L), partial CSB-YP006912BO
CSB-EP006912BO
CSB-BP006912BO
CSB-MP006912BO
CSB-EP006912BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Mitochondrial 2-oxodicarboxylate carrier (SLC25A21), partial CSB-YP021490BO1
CSB-EP021490BO1
CSB-BP021490BO1
CSB-MP021490BO1
CSB-EP021490BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine PAX-interacting protein 1 (PAXIP1), partial CSB-YP017496BO
CSB-EP017496BO
CSB-BP017496BO
CSB-MP017496BO
CSB-EP017496BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Zinc finger protein 227 (ZNF227), partial CSB-YP026595BO
CSB-EP026595BO
CSB-BP026595BO
CSB-MP026595BO
CSB-EP026595BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Phosphatidate cytidylyltransferase 2 (CDS2), partial CSB-YP005123BO1
CSB-EP005123BO1
CSB-BP005123BO1
CSB-MP005123BO1
CSB-EP005123BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine R3H domain-containing protein 2 (R3HDM2), partial CSB-YP019150BO
CSB-EP019150BO
CSB-BP019150BO
CSB-MP019150BO
CSB-EP019150BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Interferon regulatory factor 1 (IRF1) CSB-YP011814PI
CSB-EP011814PI
CSB-BP011814PI
CSB-MP011814PI
CSB-EP011814PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acyl-CoA-binding domain-containing protein 5 (Acbd5), partial CSB-YP001138RA1
CSB-EP001138RA1
CSB-BP001138RA1
CSB-MP001138RA1
CSB-EP001138RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat General transcription factor IIH subunit 2 (Gtf2h2) CSB-YP010008RA
CSB-EP010008RA
CSB-BP010008RA
CSB-MP010008RA
CSB-EP010008RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Carbonic anhydrase-related protein 10 (CA10) CSB-YP004365BO
CSB-EP004365BO
CSB-BP004365BO
CSB-MP004365BO
CSB-EP004365BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Splicing factor 3B subunit 3 (SF3B3), partial CSB-YP021127BO
CSB-BP021127BO
CSB-MP021127BO
CSB-EP021127BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine E3 ubiquitin-protein ligase MARCH3 (MARCH3), partial CSB-YP013486BO1
CSB-EP013486BO1
CSB-BP013486BO1
CSB-MP013486BO1
CSB-EP013486BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Zinc finger protein 238 (ZNF238), partial CSB-YP026604BO
CSB-EP026604BO
CSB-BP026604BO
CSB-MP026604BO
CSB-EP026604BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Polycomb group RING finger protein 5 (PCGF5) CSB-YP017608BO
CSB-EP017608BO
CSB-BP017608BO
CSB-MP017608BO
CSB-EP017608BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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