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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O45:K1 D-amino acid dehydrogenase small subunit (dadA) CSB-YP482681EOK
CSB-EP482681EOK
CSB-BP482681EOK
CSB-MP482681EOK
CSB-EP482681EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP482682EOK
CSB-EP482682EOK
CSB-BP482682EOK
CSB-MP482682EOK
CSB-EP482682EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Regulatory protein recX (recX) CSB-YP482683EOK
CSB-EP482683EOK
CSB-BP482683EOK
CSB-MP482683EOK
CSB-EP482683EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP482684EOK
CSB-EP482684EOK
CSB-BP482684EOK
CSB-MP482684EOK
CSB-EP482684EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Sulfite reductase [NADPH] hemoprotein beta-component (cysI) CSB-YP482685EOK
CSB-EP482685EOK
CSB-BP482685EOK
CSB-MP482685EOK
CSB-EP482685EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Uncharacterized protein YjeA (yjeA) CSB-YP482686EOK
CSB-EP482686EOK
CSB-BP482686EOK
CSB-MP482686EOK
CSB-EP482686EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 UPF0225 protein ychJ (ychJ) CSB-YP482687EOK
CSB-EP482687EOK
CSB-BP482687EOK
CSB-MP482687EOK
CSB-EP482687EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Enolase (eno) CSB-YP482688EOK
CSB-EP482688EOK
CSB-BP482688EOK
CSB-MP482688EOK
CSB-EP482688EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP482689EOK
CSB-EP482689EOK
CSB-BP482689EOK
CSB-MP482689EOK
CSB-EP482689EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 HTH-type transcriptional repressor NsrR (nsrR) CSB-YP482690EOK
CSB-EP482690EOK
CSB-BP482690EOK
CSB-MP482690EOK
CSB-EP482690EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Primosomal replication protein n (priB) CSB-YP482691EOK
CSB-EP482691EOK
CSB-BP482691EOK
CSB-MP482691EOK
CSB-EP482691EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP482692EOK
CSB-EP482692EOK
CSB-BP482692EOK
CSB-MP482692EOK
CSB-EP482692EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Mannonate dehydratase (uxuA) CSB-YP482693EOK
CSB-EP482693EOK
CSB-BP482693EOK
CSB-MP482693EOK
CSB-EP482693EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Altronate oxidoreductase (uxaB) CSB-YP482694EOK
CSB-EP482694EOK
CSB-BP482694EOK
CSB-MP482694EOK
CSB-EP482694EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Deoxyribose-phosphate aldolase (deoC) CSB-YP482695EOK
CSB-EP482695EOK
CSB-BP482695EOK
CSB-MP482695EOK
CSB-EP482695EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O45:K1 Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP482696EOK
CSB-EP482696EOK
CSB-BP482696EOK
CSB-MP482696EOK
CSB-EP482696EOK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Non-specific ribonucleoside hydrolase rihC (rihC) CSB-YP482697EOP
CSB-EP482697EOP
CSB-BP482697EOP
CSB-MP482697EOP
CSB-EP482697EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 L-carnitine/gamma-butyrobetaine antiporter (caiT), partial CSB-YP482698EOP
CSB-EP482698EOP
CSB-BP482698EOP
CSB-MP482698EOP
CSB-EP482698EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA) CSB-YP482699EOP
CSB-EP482699EOP
CSB-BP482699EOP
CSB-MP482699EOP
CSB-EP482699EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O81 Protein MraZ (mraZ) CSB-YP482700EOP
CSB-EP482700EOP
CSB-BP482700EOP
CSB-MP482700EOP
CSB-EP482700EOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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