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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Uncharacterized protein C9orf170 (C9orf170) CSB-YP004302HU
CSB-EP004302HU
CSB-BP004302HU
CSB-MP004302HU
CSB-EP004302HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Testis-specific XK-related protein, Y-linked 2 (XKRY2) CSB-YP026208HU
CSB-EP026208HU
CSB-BP026208HU
CSB-MP026208HU
CSB-EP026208HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 89 (TMEM89), partial CSB-YP023893HU1
CSB-EP023893HU1
CSB-BP023893HU1
CSB-MP023893HU1
CSB-EP023893HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 131-like (KIAA0922), partial CSB-YP012218HU
CSB-EP012218HU
CSB-BP012218HU
CSB-MP012218HU
CSB-EP012218HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Vacuolar ATPase assembly integral membrane protein VMA21 (VMA21), partial CSB-YP025866BO1
CSB-EP025866BO1
CSB-BP025866BO1
CSB-MP025866BO1
CSB-EP025866BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Transmembrane protein 184B (TMEM184B), partial CSB-YP023770BO1
CSB-EP023770BO1
CSB-BP023770BO1
CSB-MP023770BO1
CSB-EP023770BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Integral membrane protein 2C (ITM2C), partial CSB-YP011906BO1
CSB-EP011906BO1
CSB-BP011906BO1
CSB-MP011906BO1
CSB-EP011906BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine E3 ubiquitin-protein ligase BRE1A (RNF20), partial CSB-YP019869BO
CSB-EP019869BO
CSB-BP019869BO
CSB-MP019869BO
CSB-EP019869BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Neurolysin, mitochondrial (NLN), partial CSB-YP015860BO
CSB-EP015860BO
CSB-BP015860BO
CSB-MP015860BO
CSB-EP015860BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Selenocysteine lyase (SCLY) CSB-YP020827BO
CSB-EP020827BO
CSB-BP020827BO
CSB-MP020827BO
CSB-EP020827BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein sprouty homolog 4 (SPRY4) CSB-YP022624BO
CSB-EP022624BO
CSB-BP022624BO
CSB-MP022624BO
CSB-EP022624BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Serine/threonine-protein kinase 38 (STK38) CSB-YP022852BO
CSB-EP022852BO
CSB-BP022852BO
CSB-MP022852BO
CSB-EP022852BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine N-terminal EF-hand calcium-binding protein 3 (NECAB3) CSB-YP015676BO
CSB-EP015676BO
CSB-BP015676BO
CSB-MP015676BO
CSB-EP015676BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Charged multivesicular body protein 4a (CHMP4A) CSB-YP005362BO
CSB-EP005362BO
CSB-BP005362BO
CSB-MP005362BO
CSB-EP005362BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Myocyte-specific enhancer factor 2A (MEF2A) CSB-YP013670BO
CSB-EP013670BO
CSB-BP013670BO
CSB-MP013670BO
CSB-EP013670BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Vesicle-associated membrane protein-associated protein B (VAPB), partial CSB-YP025790BO1
CSB-EP025790BO1
CSB-BP025790BO1
CSB-MP025790BO1
CSB-EP025790BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein MAL2 (MAL2), partial CSB-YP013373BO1
CSB-EP013373BO1
CSB-BP013373BO1
CSB-MP013373BO1
CSB-EP013373BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Adropin (ENHO) CSB-YP007669BO
CSB-EP007669BO
CSB-BP007669BO
CSB-MP007669BO
CSB-EP007669BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine DNA replication complex GINS protein SLD5 (GINS4) CSB-YP009433BO
CSB-EP009433BO
CSB-BP009433BO
CSB-MP009433BO
CSB-EP009433BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine ADP-dependent glucokinase (ADPGK) CSB-YP001380BO
CSB-EP001380BO
CSB-BP001380BO
CSB-MP001380BO
CSB-EP001380BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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