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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bovine Keratin, type I cytoskeletal 40 (KRT40) CSB-YP012557BO
CSB-EP012557BO
CSB-BP012557BO
CSB-MP012557BO
CSB-EP012557BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Transcription activator BRG1 (SMARCA4), partial CSB-YP021801BO
CSB-EP021801BO
CSB-BP021801BO
CSB-MP021801BO
CSB-EP021801BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Transmembrane gamma-carboxyglutamic acid protein 1 (PRRG1), partial CSB-YP018800BO1
CSB-EP018800BO1
CSB-BP018800BO1
CSB-MP018800BO1
CSB-EP018800BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Sodium/myo-inositol cotransporter 2 (SLC5A11), partial CSB-YP021677PI
CSB-EP021677PI
CSB-BP021677PI
CSB-MP021677PI
CSB-EP021677PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Low affinity cationic amino acid transporter 2 (SLC7A2), partial CSB-YP021714PI
CSB-EP021714PI
CSB-BP021714PI
CSB-MP021714PI
CSB-EP021714PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 785 (ZNF785) CSB-YP027041HU
CSB-EP027041HU
CSB-BP027041HU
CSB-MP027041HU
CSB-EP027041HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein arginine N-methyltransferase 5 (PRMT5), partial CSB-YP018734BO
CSB-EP018734BO
CSB-BP018734BO
CSB-MP018734BO
CSB-EP018734BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine BTB/POZ domain-containing protein KCTD19 (KCTD19), partial CSB-YP012115BO
CSB-EP012115BO
CSB-BP012115BO
CSB-MP012115BO
CSB-EP012115BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Proteinase-activated receptor 1 (F2R), partial CSB-YP007924BO1
CSB-EP007924BO1
CSB-BP007924BO1
CSB-MP007924BO1
CSB-EP007924BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine 3-hydroxyacyl-CoA dehydratase 3 (PTPLAD1), partial CSB-YP019019BO1
CSB-EP019019BO1
CSB-BP019019BO1
CSB-MP019019BO1
CSB-EP019019BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Protein phosphatase 1 regulatory subunit 37 (PPP1R37), partial CSB-YP013164BO
CSB-EP013164BO
CSB-BP013164BO
CSB-MP013164BO
CSB-EP013164BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine WAS protein family homolog 1 (WASH1) CSB-YP025972BO
CSB-EP025972BO
CSB-BP025972BO
CSB-MP025972BO
CSB-EP025972BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Thyroid adenoma-associated protein homolog (Thada), partial CSB-YP023473MO
CSB-EP023473MO
CSB-BP023473MO
CSB-MP023473MO
CSB-EP023473MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Radial spoke head protein 3 homolog (RSPH3), partial CSB-YP020547BO
CSB-EP020547BO
CSB-BP020547BO
CSB-MP020547BO
CSB-EP020547BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative homeobox protein Meis3-like 2 (MEIS3P2) CSB-YP013690HU
CSB-EP013690HU
CSB-BP013690HU
CSB-MP013690HU
CSB-EP013690HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Ribonuclease P protein subunit p40 (RPP40) CSB-YP020351BO
CSB-EP020351BO
CSB-BP020351BO
CSB-MP020351BO
CSB-EP020351BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Surfeit locus protein 4 (SURF4), partial CSB-YP022965BO1
CSB-EP022965BO1
CSB-BP022965BO1
CSB-MP022965BO1
CSB-EP022965BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Nucleoporin SEH1 (SEH1L) CSB-YP020972BO
CSB-EP020972BO
CSB-BP020972BO
CSB-MP020972BO
CSB-EP020972BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Ubiquitin carboxyl-terminal hydrolase 20 (USP20), partial CSB-YP025711BO
CSB-EP025711BO
CSB-BP025711BO
CSB-MP025711BO
CSB-EP025711BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Thyroid adenoma-associated protein homolog (THADA), partial CSB-YP023473DO
CSB-EP023473DO
CSB-BP023473DO
CSB-MP023473DO
CSB-EP023473DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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