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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rat Bcl2 antagonist of cell death (Bad) CSB-YP002528RA
CSB-EP002528RA
CSB-BP002528RA
CSB-MP002528RA
CSB-EP002528RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse BET1-like protein (Bet1l), partial CSB-YP002671MO1
CSB-EP002671MO1
CSB-BP002671MO1
CSB-MP002671MO1
CSB-EP002671MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Potassium voltage-gated channel subfamily S member 1 (Kcns1), partial CSB-YP012095MO1
CSB-EP012095MO1
CSB-BP012095MO1
CSB-MP012095MO1
CSB-EP012095MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pro-neuregulin-3, membrane-bound isoform (Nrg3), partial CSB-YP016079MO1
CSB-EP016079MO1
CSB-BP016079MO1
CSB-MP016079MO1
CSB-EP016079MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Class E basic helix-loop-helix protein 40 (Bhlhe40) CSB-YP002691MO
CSB-EP002691MO
CSB-BP002691MO
CSB-MP002691MO
CSB-EP002691MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cyclin-dependent kinase 2-associated protein 1 (Cdk2ap1) CSB-YP005062MO
CSB-EP005062MO
CSB-BP005062MO
CSB-MP005062MO
CSB-EP005062MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Kinesin-like protein KIFC3 (Kifc3), partial CSB-YP012350MO
CSB-EP012350MO
CSB-BP012350MO
CSB-MP012350MO
CSB-EP012350MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Polycystin-2 (Pkd2), partial CSB-YP018061MO
CSB-EP018061MO
CSB-BP018061MO
CSB-MP018061MO
CSB-EP018061MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neurabin-2 (Ppp1r9b), partial CSB-YP018558RA
CSB-EP018558RA
CSB-BP018558RA
CSB-MP018558RA
CSB-EP018558RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 (Dhx15) CSB-YP006870MO
CSB-EP006870MO
CSB-BP006870MO
CSB-MP006870MO
CSB-EP006870MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcriptional activator protein Pur-beta (Purb) CSB-YP019084MO
CSB-EP019084MO
CSB-BP019084MO
CSB-MP019084MO
CSB-EP019084MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Monocarboxylate transporter 3 (Slc16a8), partial CSB-YP021415MO
CSB-EP021415MO
CSB-BP021415MO
CSB-MP021415MO
CSB-EP021415MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Pre-B-cell leukemia transcription factor 3 (Pbx3) CSB-YP017507MO
CSB-EP017507MO
CSB-BP017507MO
CSB-MP017507MO
CSB-EP017507MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Importin subunit alpha-4 (Kpna4) CSB-YP012486MO
CSB-EP012486MO
CSB-BP012486MO
CSB-MP012486MO
CSB-EP012486MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Acetylcholinesterase collagenic tail peptide (Colq) CSB-YP005767MO
CSB-EP005767MO
CSB-BP005767MO
CSB-MP005767MO
CSB-EP005767MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Neuropilin-2 (Nrp2), partial CSB-YP016092MO
CSB-EP016092MO
CSB-BP016092MO
CSB-MP016092MO
CSB-EP016092MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse HCLS1-associated protein X-1 (Hax1) CSB-YP010146MO
CSB-EP010146MO
CSB-BP010146MO
CSB-MP010146MO
CSB-EP010146MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Prenylated Rab acceptor protein 1 (Rabac1), partial CSB-YP019227RA1
CSB-EP019227RA1
CSB-BP019227RA1
CSB-MP019227RA1
CSB-EP019227RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Phospholipase D3 (Pld3), partial CSB-YP018146MO1
CSB-EP018146MO1
CSB-BP018146MO1
CSB-MP018146MO1
CSB-EP018146MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Amyloid beta A4 precursor protein-binding family A member 2 (Apba2) CSB-YP001887RA
CSB-EP001887RA
CSB-BP001887RA
CSB-MP001887RA
CSB-EP001887RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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