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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Proteasome subunit alpha type-7 (PSMA7) CSB-YP018873HU
CSB-EP018873HU
CSB-BP018873HU
CSB-MP018873HU
CSB-EP018873HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phytanoyl-CoA dioxygenase, peroxisomal (PHYH) CSB-YP017946HU
CSB-BP017946HU
CSB-MP017946HU
CSB-EP017946HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Interferon-inducible protein AIM2 (AIM2) CSB-YP001499HU
CSB-EP001499HU
CSB-BP001499HU
CSB-MP001499HU
CSB-EP001499HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Microsomal glutathione S-transferase 3 (MGST3), partial CSB-YP013793HU1
CSB-EP013793HU1
CSB-BP013793HU1
CSB-MP013793HU1
CSB-EP013793HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Krueppel-like factor 11 (KLF11) CSB-YP012385HU
CSB-EP012385HU
CSB-BP012385HU
CSB-MP012385HU
CSB-EP012385HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein lin-7 homolog A (LIN7A) CSB-YP012963HU
CSB-EP012963HU
CSB-BP012963HU
CSB-MP012963HU
CSB-EP012963HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitochondrial import inner membrane translocase subunit Tim23 (TIMM23), partial CSB-YP023553HU
CSB-BP023553HU
CSB-MP023553HU
CSB-EP023553HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin/ISG15-conjugating enzyme E2 L6 (UBE2L6) CSB-YP025466HU
CSB-EP025466HU
CSB-BP025466HU
CSB-MP025466HU
CSB-EP025466HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytochrome b-c1 complex subunit 8 (UQCRQ) CSB-YP025673HU
CSB-EP025673HU
CSB-BP025673HU
CSB-MP025673HU
CSB-EP025673HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) CSB-YP010329HU
CSB-EP010329HU
CSB-BP010329HU
CSB-MP010329HU
CSB-EP010329HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein phosphatase 1 regulatory subunit 12A (PPP1R12A), partial CSB-YP018511HU
CSB-EP018511HU
CSB-BP018511HU
CSB-MP018511HU
CSB-EP018511HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Heterogeneous nuclear ribonucleoprotein D-like (HNRPDL) CSB-YP010628HU
CSB-EP010628HU
CSB-BP010628HU
CSB-MP010628HU
CSB-EP010628HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative type-1 protein phosphatase inhibitor 4 (PPP1R2P9) CSB-YP018546HU
CSB-EP018546HU
CSB-BP018546HU
CSB-MP018546HU
CSB-EP018546HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tripartite motif-containing protein 66 (TRIM66), partial CSB-YP024505HU
CSB-EP024505HU
CSB-BP024505HU
CSB-MP024505HU
CSB-EP024505HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Plexin-B2 (PLXNB2), partial CSB-YP018223HU
CSB-EP018223HU
CSB-BP018223HU
CSB-MP018223HU
CSB-EP018223HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tectonin beta-propeller repeat-containing protein 2 (TECPR2), partial CSB-YP023369HU
CSB-EP023369HU
CSB-BP023369HU
CSB-MP023369HU
CSB-EP023369HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Synemin (SYNM), partial CSB-YP023019HU
CSB-EP023019HU
CSB-BP023019HU
CSB-MP023019HU
CSB-EP023019HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A (ANKRD28), partial CSB-YP001766HU
CSB-EP001766HU
CSB-BP001766HU
CSB-MP001766HU
CSB-EP001766HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit alpha (CHUK) CSB-YP005420HU
CSB-EP005420HU
CSB-BP005420HU
CSB-MP005420HU
CSB-EP005420HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), partial CSB-YP001450HU1
CSB-EP001450HU1
CSB-BP001450HU1
CSB-MP001450HU1
CSB-EP001450HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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