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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse A-kinase anchor protein 3 (Akap3), partial CSB-YP001525MO
CSB-EP001525MO
CSB-BP001525MO
CSB-MP001525MO
CSB-EP001525MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Dihydropyrimidine dehydrogenase [NADP (+)] (Dpyd), partial CSB-YP007168RA
CSB-EP007168RA
CSB-BP007168RA
CSB-MP007168RA
CSB-EP007168RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Myocyte-specific enhancer factor 2D (Mef2d) CSB-YP013674RA
CSB-EP013674RA
CSB-BP013674RA
CSB-MP013674RA
CSB-EP013674RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat DNA primase large subunit (Prim2) CSB-YP018681RA
CSB-EP018681RA
CSB-BP018681RA
CSB-MP018681RA
CSB-EP018681RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Integral membrane protein 2B (Itm2b), partial CSB-YP011904MO1
CSB-EP011904MO1
CSB-BP011904MO1
CSB-MP011904MO1
CSB-EP011904MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C-C motif chemokine 20 (Ccl20) CSB-YP004784MO
CSB-EP004784MO
CSB-BP004784MO
CSB-MP004784MO
CSB-EP004784MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Synaptophysin-like protein 2 (Sypl2), partial CSB-YP023026MO1
CSB-EP023026MO1
CSB-BP023026MO1
CSB-MP023026MO1
CSB-EP023026MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DnaJ homolog subfamily B member 5 (Dnajb5) CSB-YP007011MO
CSB-EP007011MO
CSB-BP007011MO
CSB-MP007011MO
CSB-EP007011MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken WD repeat-containing protein 1 (WDR1), partial CSB-YP025995CH
CSB-EP025995CH
CSB-BP025995CH
CSB-MP025995CH
CSB-EP025995CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Cyclic AMP-dependent transcription factor ATF-2 (ATF2) CSB-YP002270CH
CSB-EP002270CH
CSB-BP002270CH
CSB-MP002270CH
CSB-EP002270CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transient receptor potential cation channel subfamily M member 2 (TRPM2), partial CSB-YP025084HU
CSB-EP025084HU
CSB-BP025084HU
CSB-MP025084HU
CSB-EP025084HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Contactin-5 (CNTN5), partial CSB-YP005690HU
CSB-EP005690HU
CSB-BP005690HU
CSB-MP005690HU
CSB-EP005690HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Actin-like protein 6B (ACTL6B) CSB-YP001237HU
CSB-BP001237HU
CSB-MP001237HU
CSB-EP001237HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (GFPT2) CSB-YP009378HU
CSB-EP009378HU
CSB-BP009378HU
CSB-MP009378HU
CSB-EP009378HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Slit homolog 2 protein (SLIT2), partial CSB-YP021768HU
CSB-EP021768HU
CSB-BP021768HU
CSB-MP021768HU
CSB-EP021768HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin-protein ligase listerin (LTN1), partial CSB-YP026646HU
CSB-EP026646HU
CSB-BP026646HU
CSB-MP026646HU
CSB-EP026646HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Importin-13 (IPO13), partial CSB-YP011776HU
CSB-EP011776HU
CSB-BP011776HU
CSB-MP011776HU
CSB-EP011776HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rho-related BTB domain-containing protein 1 (RHOBTB1) CSB-YP019684HU
CSB-EP019684HU
CSB-BP019684HU
CSB-MP019684HU
CSB-EP019684HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein transport protein Sec24D (SEC24D), partial CSB-YP020953HU
CSB-EP020953HU
CSB-BP020953HU
CSB-MP020953HU
CSB-EP020953HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 UFM1-protein ligase 1 (UFL1) CSB-YP012209HU
CSB-EP012209HU
CSB-BP012209HU
CSB-MP012209HU
CSB-EP012209HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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