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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Apolipoprotein L3 (APOL3) CSB-YP001942HU
CSB-EP001942HU
CSB-BP001942HU
CSB-MP001942HU
CSB-EP001942HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Interleukin-18 receptor accessory protein (IL18RAP), partial CSB-YP011611HU1
CSB-EP011611HU1
CSB-BP011611HU1
CSB-MP011611HU1
CSB-EP011611HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Brain mitochondrial carrier protein 1 (SLC25A14), partial CSB-YP021480HU1
CSB-EP021480HU1
CSB-BP021480HU1
CSB-MP021480HU1
CSB-EP021480HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RAS guanyl-releasing protein 1 (RASGRP1), partial CSB-YP019362HU
CSB-EP019362HU
CSB-BP019362HU
CSB-MP019362HU
CSB-EP019362HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ly6/PLAUR domain-containing protein 3 (LYPD3) CSB-YP013263HU
CSB-BP013263HU
CSB-MP013263HU
CSB-EP013263HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Vesicle-associated membrane protein-associated protein B/C (VAPB), partial CSB-YP025790HU1
CSB-EP025790HU1
CSB-BP025790HU1
CSB-MP025790HU1
CSB-EP025790HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SNARE-associated protein Snapin (SNAPIN) CSB-YP021882HU
CSB-EP021882HU
CSB-BP021882HU
CSB-MP021882HU
CSB-EP021882HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (NDUFA10) CSB-YP015619HU
CSB-EP015619HU
CSB-BP015619HU
CSB-MP015619HU
CSB-EP015619HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Homeobox protein SIX3 (SIX3) CSB-YP021350HU
CSB-EP021350HU
CSB-BP021350HU
CSB-MP021350HU
CSB-EP021350HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human E3 ubiquitin-protein ligase ARIH2 (ARIH2) CSB-YP002069HU
CSB-EP002069HU
CSB-BP002069HU
CSB-MP002069HU
CSB-EP002069HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mitogen-activated protein kinase kinase kinase 6 (MAP3K6), partial CSB-YP013428HU
CSB-EP013428HU
CSB-BP013428HU
CSB-MP013428HU
CSB-EP013428HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pre-mRNA-splicing factor SLU7 (SLU7) CSB-YP021783HU
CSB-EP021783HU
CSB-BP021783HU
CSB-MP021783HU
CSB-EP021783HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Adenylyltransferase and sulfurtransferase MOCS3 (MOCS3) CSB-YP014707HU
CSB-EP014707HU
CSB-BP014707HU
CSB-MP014707HU
CSB-EP014707HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mediator of RNA polymerase II transcription subunit 26 (MED26) CSB-YP013659HU
CSB-EP013659HU
CSB-BP013659HU
CSB-MP013659HU
CSB-EP013659HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein ZIC 2 (ZIC2) CSB-YP026485HU
CSB-EP026485HU
CSB-BP026485HU
CSB-MP026485HU
CSB-EP026485HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Supervillin (SVIL), partial CSB-YP022982HU
CSB-EP022982HU
CSB-BP022982HU
CSB-MP022982HU
CSB-EP022982HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) CSB-YP001483HU
CSB-BP001483HU
CSB-MP001483HU
CSB-EP001483HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Lebercilin-like protein (LCA5L) CSB-YP012782HU
CSB-EP012782HU
CSB-BP012782HU
CSB-MP012782HU
CSB-EP012782HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Proteasome assembly chaperone 1 (PSMG1) CSB-YP018921HU
CSB-EP018921HU
CSB-BP018921HU
CSB-MP018921HU
CSB-EP018921HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Neuroendocrine secretory protein 55 (GNAS) CSB-YP009596HU
CSB-BP009596HU
CSB-MP009596HU
CSB-EP009596HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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