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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa Choline dehydrogenase (betA), partial CSB-YP005335EZY
CSB-EP005335EZY
CSB-BP005335EZY
CSB-MP005335EZY
CSB-EP005335EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121EZY
CSB-EP010121EZY
CSB-BP010121EZY
CSB-MP010121EZY
CSB-EP010121EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transthyretin (ttr) CSB-EP025270XBE
CSB-BP025270XBE
CSB-MP025270XBE
CSB-EP025270XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transmembrane protein 92 (Tmem92), partial CSB-YP023897MO1
CSB-EP023897MO1
CSB-BP023897MO1
CSB-MP023897MO1
CSB-EP023897MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Histidine ammonia-lyase (hutH), partial CSB-YP010123SYW
CSB-EP010123SYW
CSB-BP010123SYW
CSB-MP010123SYW
CSB-EP010123SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum CTP synthase (pyrG), partial CSB-YP006176BXA
CSB-EP006176BXA
CSB-BP006176BXA
CSB-MP006176BXA
CSB-EP006176BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ornithine carbamoyltransferase (arcB) CSB-YP017270BWY
CSB-EP017270BWY
CSB-BP017270BWY
CSB-MP017270BWY
CSB-EP017270BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4a Uroporphyrinogen decarboxylase (hemE) CSB-YP025678LPW
CSB-EP025678LPW
CSB-BP025678LPW
CSB-MP025678LPW
CSB-EP025678LPW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Uroporphyrinogen decarboxylase (hemE) CSB-YP025678SYS
CSB-EP025678SYS
CSB-BP025678SYS
CSB-MP025678SYS
CSB-EP025678SYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Thiopurine S-methyltransferase (tpm) CSB-YP024110SYS
CSB-EP024110SYS
CSB-BP024110SYS
CSB-MP024110SYS
CSB-EP024110SYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroflexus aggregans CTP synthase (pyrG), partial CSB-YP006176DSV
CSB-EP006176DSV
CSB-BP006176DSV
CSB-MP006176DSV
CSB-EP006176DSV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121EPV
CSB-EP010121EPV
CSB-BP010121EPV
CSB-MP010121EPV
CSB-EP010121EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum GTP cyclohydrolase 1 (folE) CSB-YP009317DUL
CSB-EP009317DUL
CSB-BP009317DUL
CSB-MP009317DUL
CSB-EP009317DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Histidine ammonia-lyase (hutH), partial CSB-YP010123MTB
CSB-EP010123MTB
CSB-BP010123MTB
CSB-MP010123MTB
CSB-EP010123MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans CTP synthase (pyrG), partial CSB-YP006176MTB
CSB-EP006176MTB
CSB-BP006176MTB
CSB-MP006176MTB
CSB-EP006176MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Uroporphyrinogen decarboxylase (hemE) CSB-YP025678EZY
CSB-EP025678EZY
CSB-BP025678EZY
CSB-MP025678EZY
CSB-EP025678EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Histidine ammonia-lyase (hutH), partial CSB-YP010123EZY
CSB-EP010123EZY
CSB-BP010123EZY
CSB-MP010123EZY
CSB-EP010123EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Argininosuccinate lyase (argH) CSB-YP002213EZY
CSB-EP002213EZY
CSB-BP002213EZY
CSB-MP002213EZY
CSB-EP002213EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Adenine phosphoribosyltransferase (apt) CSB-YP001954SYW
CSB-EP001954SYW
CSB-BP001954SYW
CSB-MP001954SYW
CSB-EP001954SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum 6-phosphogluconolactonase (pgl) CSB-YP017861BXA
CSB-EP017861BXA
CSB-BP017861BXA
CSB-MP017861BXA
CSB-EP017861BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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