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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pneumoniae Uracil-DNA glycosylase (ung) CSB-YP025641FNA
CSB-EP025641FNA
CSB-BP025641FNA
CSB-MP025641FNA
CSB-EP025641FNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachipleistophora hominis Frataxin (YFH1) CSB-YP009086TOX
CSB-EP009086TOX
CSB-BP009086TOX
CSB-MP009086TOX
CSB-EP009086TOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Choline dehydrogenase (betA), partial CSB-YP005335AWQ
CSB-EP005335AWQ
CSB-BP005335AWQ
CSB-MP005335AWQ
CSB-EP005335AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii CTP synthase (pyrG), partial CSB-YP006176AWQ
CSB-EP006176AWQ
CSB-BP006176AWQ
CSB-MP006176AWQ
CSB-EP006176AWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Uracil-DNA glycosylase (ung) CSB-YP025641AWP
CSB-EP025641AWP
CSB-BP025641AWP
CSB-MP025641AWP
CSB-EP025641AWP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus somnus Adenine phosphoribosyltransferase (apt) CSB-YP001954HTE
CSB-EP001954HTE
CSB-BP001954HTE
CSB-MP001954HTE
CSB-EP001954HTE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella halifaxensis Aminomethyltransferase (gcvT) CSB-YP001684SYU
CSB-EP001684SYU
CSB-BP001684SYU
CSB-MP001684SYU
CSB-EP001684SYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella halifaxensis Uroporphyrinogen decarboxylase (hemE) CSB-YP025678SYU
CSB-EP025678SYU
CSB-BP025678SYU
CSB-MP025678SYU
CSB-EP025678SYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira biflexa serovar Patoc Spermidine synthase (speE) CSB-YP022668LOR
CSB-EP022668LOR
CSB-BP022668LOR
CSB-MP022668LOR
CSB-EP022668LOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira biflexa serovar Patoc Aminomethyltransferase (gcvT) CSB-YP001684LOR
CSB-EP001684LOR
CSB-BP001684LOR
CSB-MP001684LOR
CSB-EP001684LOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transmembrane protein 71 (tmem71), partial CSB-YP023875DIL1
CSB-EP023875DIL1
CSB-BP023875DIL1
CSB-MP023875DIL1
CSB-EP023875DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Spermidine synthase (speE) CSB-YP022668XAZ
CSB-EP022668XAZ
CSB-BP022668XAZ
CSB-MP022668XAZ
CSB-EP022668XAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris CTP synthase (pyrG), partial CSB-YP006176XAZ
CSB-EP006176XAZ
CSB-BP006176XAZ
CSB-MP006176XAZ
CSB-EP006176XAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum CTP synthase (pyrG), partial CSB-YP006176HTL
CSB-EP006176HTL
CSB-BP006176HTL
CSB-MP006176HTL
CSB-EP006176HTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Uroporphyrinogen decarboxylase (hemE) CSB-YP025678FGA
CSB-EP025678FGA
CSB-BP025678FGA
CSB-MP025678FGA
CSB-EP025678FGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Transmembrane protein 218 (tmem218), partial CSB-YP023812XBF1
CSB-EP023812XBF1
CSB-BP023812XBF1
CSB-MP023812XBF1
CSB-EP023812XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis Adenine phosphoribosyltransferase (apt) CSB-YP001954BWU
CSB-EP001954BWU
CSB-BP001954BWU
CSB-MP001954BWU
CSB-EP001954BWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acaryochloris marina Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121AVV
CSB-EP010121AVV
CSB-BP010121AVV
CSB-MP010121AVV
CSB-EP010121AVV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 3 Cytidine deaminase (cdd) CSB-YP004976AXH
CSB-EP004976AXH
CSB-BP004976AXH
CSB-MP004976AXH
CSB-EP004976AXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ornithine carbamoyltransferase (argI) CSB-YP017270ENP
CSB-EP017270ENP
CSB-BP017270ENP
CSB-MP017270ENP
CSB-EP017270ENP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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