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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant DNA-directed RNA polymerase I subunit RPA1 (TRP11), partial CSB-YP018322TQV
CSB-EP018322TQV
CSB-BP018322TQV
CSB-MP018322TQV
CSB-EP018322TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pomatostomus superciliosus Cytochrome b (MT-CYB), partial CSB-YP015075PPL1
CSB-EP015075PPL1
CSB-BP015075PPL1
CSB-MP015075PPL1
CSB-EP015075PPL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Phosphatidylinositol transfer protein alpha isoform (Pitpna) CSB-YP018035RA
CSB-EP018035RA
CSB-BP018035RA
CSB-MP018035RA
CSB-EP018035RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Interleukin-1 alpha (Il1a) CSB-YP011613RA
CSB-EP011613RA
CSB-BP011613RA
CSB-MP011613RA
CSB-EP011613RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Arginyl-tRNA--protein transferase 1 (ATE1), partial CSB-YP002268SVG
CSB-EP002268SVG
CSB-BP002268SVG
CSB-MP002268SVG
CSB-EP002268SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lithobates catesbeiana Cytochrome b (mt-cyb), partial CSB-YP015075LQA
CSB-EP015075LQA
CSB-BP015075LQA
CSB-MP015075LQA
CSB-EP015075LQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multidrug resistance protein 3 (MDR3) CSB-YP001050EKM
CSB-EP001050EKM
CSB-BP001050EKM
CSB-MP001050EKM
CSB-EP001050EKM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat SPARC (Sparc) CSB-YP022486RA
CSB-EP022486RA
CSB-BP022486RA
CSB-MP022486RA
CSB-EP022486RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe TATA-box-binding protein (tbp1) CSB-YP023239SXV
CSB-EP023239SXV
CSB-BP023239SXV
CSB-MP023239SXV
CSB-EP023239SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain (TUBB) CSB-YP025318EEQ
CSB-EP025318EEQ
CSB-BP025318EEQ
CSB-MP025318EEQ
CSB-EP025318EEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cholesterol 7-alpha-monooxygenase (Cyp7a1) CSB-YP006460RA
CSB-EP006460RA
CSB-BP006460RA
CSB-MP006460RA
CSB-EP006460RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Separin (cut1), partial CSB-YP007826SXV
CSB-EP007826SXV
CSB-BP007826SXV
CSB-MP007826SXV
CSB-EP007826SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Elongation factor G (fusA), partial CSB-YP009373FPZ
CSB-EP009373FPZ
CSB-BP009373FPZ
CSB-MP009373FPZ
CSB-EP009373FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lymnaea stagnalis Tubulin beta chain CSB-YP025318LRQ
CSB-EP025318LRQ
CSB-BP025318LRQ
CSB-MP025318LRQ
CSB-EP025318LRQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma gallisepticum Elongation factor Tu (tuf) CSB-YP025342MWI
CSB-EP025342MWI
CSB-BP025342MWI
CSB-MP025342MWI
CSB-EP025342MWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster NADH-ubiquinone oxidoreductase chain 6 (mt:ND6), partial CSB-YP015082DLU1
CSB-EP015082DLU1
CSB-BP015082DLU1
CSB-MP015082DLU1
CSB-EP015082DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailurus fulgens Hemoglobin subunit alpha (HBA) CSB-YP010147AYY
CSB-EP010147AYY
CSB-BP010147AYY
CSB-MP010147AYY
CSB-EP010147AYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panthera pardus saxicolor Hemoglobin subunit alpha (HBA) CSB-YP010147PBG
CSB-EP010147PBG
CSB-BP010147PBG
CSB-MP010147PBG
CSB-EP010147PBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix argentata Hemoglobin subunit beta (HBB) CSB-YP010150CBA
CSB-EP010150CBA
CSB-BP010150CBA
CSB-MP010150CBA
CSB-EP010150CBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tursiops truncatus Hemoglobin subunit beta (HBB) CSB-YP010150TRF
CSB-EP010150TRF
CSB-BP010150TRF
CSB-MP010150TRF
CSB-EP010150TRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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