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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Cytochrome b-c1 complex subunit 6 (QCR6) CSB-YP025671SVG
CSB-EP025671SVG
CSB-BP025671SVG
CSB-MP025671SVG
CSB-EP025671SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome b (COB), partial CSB-YP015075SVG
CSB-EP015075SVG
CSB-BP015075SVG
CSB-MP015075SVG
CSB-EP015075SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cytochrome P450 1A1 (Cyp1a1) CSB-YP006395RA
CSB-BP006395RA
CSB-MP006395RA
CSB-EP006395RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytochrome c oxidase subunit 1 (Mtco1), partial CSB-YP015072MO
CSB-EP015072MO
CSB-BP015072MO
CSB-MP015072MO
CSB-EP015072MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Cytochrome c oxidase subunit 1 (mt:CoI), partial CSB-YP015072DLU
CSB-EP015072DLU
CSB-BP015072DLU
CSB-MP015072DLU
CSB-EP015072DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Cytochrome c oxidase subunit 2 (mt:CoII), partial CSB-YP015073DLU1
CSB-EP015073DLU1
CSB-BP015073DLU1
CSB-MP015073DLU1
CSB-EP015073DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Cytochrome c oxidase subunit 2 (COX2), partial CSB-YP015073TQN1
CSB-EP015073TQN1
CSB-BP015073TQN1
CSB-MP015073TQN1
CSB-EP015073TQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytochrome c oxidase subunit 3 (Mtco3), partial CSB-YP015074MO1
CSB-EP015074MO1
CSB-BP015074MO1
CSB-MP015074MO1
CSB-EP015074MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cytochrome c oxidase subunit 3 (mt-co3), partial CSB-YP015074XBE1
CSB-EP015074XBE1
CSB-BP015074XBE1
CSB-MP015074XBE1
CSB-EP015074XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Cytochrome c oxidase subunit 3 (cox-3), partial CSB-YP015074NHA1
CSB-EP015074NHA1
CSB-BP015074NHA1
CSB-MP015074NHA1
CSB-EP015074NHA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Superoxide dismutase [Cu-Zn] (SOD1) CSB-YP022397SVG
CSB-EP022397SVG
CSB-BP022397SVG
CSB-MP022397SVG
CSB-EP022397SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Superoxide dismutase [Mn] (sodA) CSB-YP022398GFM
CSB-EP022398GFM
CSB-BP022398GFM
CSB-MP022398GFM
CSB-EP022398GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (thyA) CSB-YP025393LMH
CSB-EP025393LMH
CSB-BP025393LMH
CSB-MP025393LMH
CSB-EP025393LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Aspartate aminotransferase (aspC) CSB-YP009681ENV
CSB-EP009681ENV
CSB-BP009681ENV
CSB-MP009681ENV
CSB-EP009681ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Epidermal growth factor receptor (EGFR), partial CSB-YP007479HU
CSB-BP007479HU
CSB-EP007479HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Phospholipase A2 CSB-YP018091DNK
CSB-BP018091DNK
CSB-MP018091DNK
CSB-EP018091DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Connochaetes taurinus Ribonuclease pancreatic (RNASE1) CSB-YP019789DVR
CSB-EP019789DVR
CSB-BP019789DVR
CSB-MP019789DVR
CSB-EP019789DVR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rangifer tarandus Ribonuclease pancreatic (RNASE1) CSB-YP019789RBL
CSB-EP019789RBL
CSB-BP019789RBL
CSB-MP019789RBL
CSB-EP019789RBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hippopotamus amphibius Ribonuclease pancreatic (RNASE1) CSB-YP019789HHI
CSB-EP019789HHI
CSB-BP019789HHI
CSB-MP019789HHI
CSB-EP019789HHI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Lysozyme C (LYZ) CSB-YP013283DXJ
CSB-EP013283DXJ
CSB-BP013283DXJ
CSB-MP013283DXJ
CSB-EP013283DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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