Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus cereus Probable multidrug resistance protein norM (norM), partial CSB-YP726952BAAD
CSB-EP726952BAAD
CSB-BP726952BAAD
CSB-MP726952BAAD
CSB-EP726952BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphonoacetaldehyde hydrolase (phnX) CSB-YP726953BAAD
CSB-EP726953BAAD
CSB-BP726953BAAD
CSB-MP726953BAAD
CSB-EP726953BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Tryptophan synthase alpha chain (trpA) CSB-YP726954BAAD
CSB-EP726954BAAD
CSB-BP726954BAAD
CSB-MP726954BAAD
CSB-EP726954BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Anthranilate phosphoribosyltransferase (trpD) CSB-YP726955BAAD
CSB-EP726955BAAD
CSB-BP726955BAAD
CSB-MP726955BAAD
CSB-EP726955BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0736 protein BCE33L1074 (BCE33L1074) CSB-YP726956BAAD
CSB-EP726956BAAD
CSB-BP726956BAAD
CSB-MP726956BAAD
CSB-EP726956BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative 3-methyladenine DNA glycosylase (BCE33L0774) CSB-YP726957BAAD
CSB-EP726957BAAD
CSB-BP726957BAAD
CSB-MP726957BAAD
CSB-EP726957BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Potassium-transporting ATPase A chain (kdpA), partial CSB-YP726958BAAD
CSB-EP726958BAAD
CSB-BP726958BAAD
CSB-MP726958BAAD
CSB-EP726958BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0295 protein BCE33L0445 (BCE33L0445), partial CSB-YP726959BAAD1
CSB-EP726959BAAD1
CSB-BP726959BAAD1
CSB-MP726959BAAD1
CSB-EP726959BAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Processive diacylglycerol glucosyltransferase (ugtP) CSB-YP726960BAAD
CSB-EP726960BAAD
CSB-BP726960BAAD
CSB-MP726960BAAD
CSB-EP726960BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Methionine import ATP-binding protein MetN 2 (metN2) CSB-YP726961BAAD
CSB-EP726961BAAD
CSB-BP726961BAAD
CSB-MP726961BAAD
CSB-EP726961BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 60 kDa chaperonin (groL), partial CSB-YP726962BAAD
CSB-EP726962BAAD
CSB-BP726962BAAD
CSB-MP726962BAAD
CSB-EP726962BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP726963BAAD
CSB-EP726963BAAD
CSB-BP726963BAAD
CSB-MP726963BAAD
CSB-EP726963BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus tRNA pseudouridine synthase A 1 (truA1) CSB-YP726964BAAD
CSB-EP726964BAAD
CSB-BP726964BAAD
CSB-MP726964BAAD
CSB-EP726964BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S11 (rpsK) CSB-YP726965BAAD
CSB-EP726965BAAD
CSB-BP726965BAAD
CSB-MP726965BAAD
CSB-EP726965BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L6 (rplF) CSB-YP726966BAAD
CSB-EP726966BAAD
CSB-BP726966BAAD
CSB-MP726966BAAD
CSB-EP726966BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L16 (rplP) CSB-YP726967BAAD
CSB-EP726967BAAD
CSB-BP726967BAAD
CSB-MP726967BAAD
CSB-EP726967BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L3 (rplC) CSB-YP726968BAAD
CSB-EP726968BAAD
CSB-BP726968BAAD
CSB-MP726968BAAD
CSB-EP726968BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S7 (rpsG) CSB-YP726969BAAD
CSB-EP726969BAAD
CSB-BP726969BAAD
CSB-MP726969BAAD
CSB-EP726969BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 33 kDa chaperonin (hslO) CSB-YP726970BAAD
CSB-EP726970BAAD
CSB-BP726970BAAD
CSB-MP726970BAAD
CSB-EP726970BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP726971BAAD
CSB-EP726971BAAD
CSB-BP726971BAAD
CSB-MP726971BAAD
CSB-EP726971BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>