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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Debaryomyces hansenii Autophagy-related protein 3 (ATG3) CSB-YP728203DIS
CSB-EP728203DIS
CSB-BP728203DIS
CSB-MP728203DIS
CSB-EP728203DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii 40S ribosomal protein S16 (RPS16) CSB-YP728204DIS
CSB-EP728204DIS
CSB-BP728204DIS
CSB-MP728204DIS
CSB-EP728204DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Nascent polypeptide-associated complex subunit alpha (EGD2) CSB-YP728205DIS
CSB-EP728205DIS
CSB-BP728205DIS
CSB-MP728205DIS
CSB-EP728205DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Sulfate adenylyltransferase (MET3), partial CSB-YP728206DIS
CSB-EP728206DIS
CSB-BP728206DIS
CSB-MP728206DIS
CSB-EP728206DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase FAL1 (FAL1) CSB-YP728207DIS
CSB-EP728207DIS
CSB-BP728207DIS
CSB-MP728207DIS
CSB-EP728207DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii mRNA-capping enzyme subunit alpha (CEG1) CSB-YP728208DIS
CSB-EP728208DIS
CSB-BP728208DIS
CSB-MP728208DIS
CSB-EP728208DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial import inner membrane translocase subunit TIM54 (TIM54), partial CSB-YP728209DIS1
CSB-EP728209DIS1
CSB-BP728209DIS1
CSB-MP728209DIS1
CSB-EP728209DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Diphthamide biosynthesis protein 3 (DPH3) CSB-YP728210DIS
CSB-EP728210DIS
CSB-BP728210DIS
CSB-MP728210DIS
CSB-EP728210DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ubiquitin-conjugating enzyme E2 2 (UBC2) CSB-YP728211DIS
CSB-EP728211DIS
CSB-BP728211DIS
CSB-MP728211DIS
CSB-EP728211DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Pre-mRNA-splicing factor CWC22 (CWC22), partial CSB-YP728212DIS
CSB-EP728212DIS
CSB-BP728212DIS
CSB-MP728212DIS
CSB-EP728212DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii 37S ribosomal protein S25, mitochondrial (RSM25) CSB-YP728213DIS
CSB-EP728213DIS
CSB-BP728213DIS
CSB-MP728213DIS
CSB-EP728213DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 12 (SRB8), partial CSB-YP728214DIS
CSB-EP728214DIS
CSB-BP728214DIS
CSB-MP728214DIS
CSB-EP728214DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii SVP1-like protein 2 (HSV2) CSB-YP728215DIS
CSB-EP728215DIS
CSB-BP728215DIS
CSB-MP728215DIS
CSB-EP728215DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase MAK5 (MAK5), partial CSB-YP728216DIS
CSB-EP728216DIS
CSB-BP728216DIS
CSB-MP728216DIS
CSB-EP728216DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Methionine aminopeptidase 2 (MAP2) CSB-YP728217DIS
CSB-EP728217DIS
CSB-BP728217DIS
CSB-MP728217DIS
CSB-EP728217DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Autophagy protein 5 (ATG5) CSB-YP728218DIS
CSB-EP728218DIS
CSB-BP728218DIS
CSB-MP728218DIS
CSB-EP728218DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Ubiquitin-related modifier 1 (URM1) CSB-YP728219DIS
CSB-EP728219DIS
CSB-BP728219DIS
CSB-MP728219DIS
CSB-EP728219DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Autophagy-related protein 9 (ATG9), partial CSB-YP728220DIS
CSB-EP728220DIS
CSB-BP728220DIS
CSB-MP728220DIS
CSB-EP728220DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii GPI ethanolamine phosphate transferase 1 (MCD4), partial CSB-YP728221DIS
CSB-EP728221DIS
CSB-BP728221DIS
CSB-MP728221DIS
CSB-EP728221DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Golgi to ER traffic protein 2 (GET2), partial CSB-YP728222DIS1
CSB-EP728222DIS1
CSB-BP728222DIS1
CSB-MP728222DIS1
CSB-EP728222DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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