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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Orgyia pseudotsugata multicapsid polyhedrosis virus Ecdysteroid UDP-glucosyltransferase (EGT) CSB-YP727442OEU
CSB-EP727442OEU
CSB-BP727442OEU
CSB-MP727442OEU
CSB-EP727442OEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bean common mosaic necrosis virus Genome polyprotein, partial CSB-YP727443BFQ
CSB-EP727443BFQ
CSB-BP727443BFQ
CSB-MP727443BFQ
CSB-EP727443BFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine ephemeral fever virus Protein alpha-3 (alpha) CSB-YP727444BJQ
CSB-EP727444BJQ
CSB-BP727444BJQ
CSB-MP727444BJQ
CSB-EP727444BJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet necrotic yellow vein virus Replicase (ORF1), partial CSB-YP727445BSO
CSB-EP727445BSO
CSB-BP727445BSO
CSB-MP727445BSO
CSB-EP727445BSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine respiratory syncytial virus Non-structural protein 2 (1B) CSB-YP727446BVL
CSB-EP727446BVL
CSB-BP727446BVL
CSB-MP727446BVL
CSB-EP727446BVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP727447RFU
CSB-EP727447RFU
CSB-BP727447RFU
CSB-MP727447RFU
CSB-EP727447RFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM154B (FAM154B) CSB-YP727448HU
CSB-EP727448HU
CSB-BP727448HU
CSB-MP727448HU
CSB-EP727448HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Maturation protein (PIVA2) CSB-YP727449CCJ
CSB-EP727449CCJ
CSB-BP727449CCJ
CSB-MP727449CCJ
CSB-EP727449CCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine adenovirus serotype 1 Early E2A DNA-binding protein (DBP) CSB-YP727450CCJ
CSB-EP727450CCJ
CSB-BP727450CCJ
CSB-MP727450CCJ
CSB-EP727450CCJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canine coronavirus Spike glycoprotein (S), partial CSB-YP727451CCP
CSB-EP727451CCP
CSB-BP727451CCP
CSB-MP727451CCP
CSB-EP727451CCP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Alpha- (1,3)-fucosyltransferase (FUT9), partial CSB-YP727452EQV
CSB-EP727452EQV
CSB-BP727452EQV
CSB-MP727452EQV
CSB-EP727452EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus Lysozyme C (LYZ) CSB-YP727453HAU
CSB-EP727453HAU
CSB-BP727453HAU
CSB-MP727453HAU
CSB-EP727453HAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 50S ribosomal protein L34 (rpmH) CSB-YP727454BQU
CSB-EP727454BQU
CSB-BP727454BQU
CSB-MP727454BQU
CSB-EP727454BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Nucleoid occlusion protein (noc) CSB-YP727455BQU
CSB-EP727455BQU
CSB-BP727455BQU
CSB-MP727455BQU
CSB-EP727455BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Beta-galactosidase ganA (ganA), partial CSB-YP727456BQU
CSB-EP727456BQU
CSB-BP727456BQU
CSB-MP727456BQU
CSB-EP727456BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase (iolD), partial CSB-YP727457BQU
CSB-EP727457BQU
CSB-BP727457BQU
CSB-MP727457BQU
CSB-EP727457BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Lantibiotic lichenicidin A1 (lanA1) CSB-YP727458BQU
CSB-EP727458BQU
CSB-BP727458BQU
CSB-MP727458BQU
CSB-EP727458BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 (murA2) CSB-YP727459BQU
CSB-EP727459BQU
CSB-BP727459BQU
CSB-MP727459BQU
CSB-EP727459BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis Chromosome-anchoring protein racA (racA) CSB-YP727460BQU
CSB-EP727460BQU
CSB-BP727460BQU
CSB-MP727460BQU
CSB-EP727460BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus licheniformis UPF0340 protein BLi03936/BL03990 (BLi03936, BL03990) CSB-YP727461BQU
CSB-EP727461BQU
CSB-BP727461BQU
CSB-MP727461BQU
CSB-EP727461BQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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