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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP730942BAAD
CSB-EP730942BAAD
CSB-BP730942BAAD
CSB-MP730942BAAD
CSB-EP730942BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus D-alanine--poly (phosphoribitol) ligase subunit 1 (dltA), partial CSB-YP730943BAAD
CSB-EP730943BAAD
CSB-BP730943BAAD
CSB-MP730943BAAD
CSB-EP730943BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lactate utilization protein B (lutB) CSB-YP730944BAAD
CSB-EP730944BAAD
CSB-BP730944BAAD
CSB-MP730944BAAD
CSB-EP730944BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Indole-3-glycerol phosphate synthase (trpC) CSB-YP730945BAAD
CSB-EP730945BAAD
CSB-BP730945BAAD
CSB-MP730945BAAD
CSB-EP730945BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Ferrochelatase 2 (hemH2) CSB-YP730946BAAD
CSB-EP730946BAAD
CSB-BP730946BAAD
CSB-MP730946BAAD
CSB-EP730946BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol kinase (glpK) CSB-YP730947BAAD
CSB-EP730947BAAD
CSB-BP730947BAAD
CSB-MP730947BAAD
CSB-EP730947BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Undecaprenyl-diphosphatase 2 (uppP2), partial CSB-YP730948BAAD1
CSB-EP730948BAAD1
CSB-BP730948BAAD1
CSB-MP730948BAAD1
CSB-EP730948BAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Regulatory protein recX (recX) CSB-YP730949BAAD
CSB-EP730949BAAD
CSB-BP730949BAAD
CSB-MP730949BAAD
CSB-EP730949BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C (gatC) CSB-YP730950BAAD
CSB-EP730950BAAD
CSB-BP730950BAAD
CSB-MP730950BAAD
CSB-EP730950BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP730951BAAD
CSB-EP730951BAAD
CSB-BP730951BAAD
CSB-MP730951BAAD
CSB-EP730951BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoglucosamine mutase (glmM) CSB-YP730952BAAD
CSB-EP730952BAAD
CSB-BP730952BAAD
CSB-MP730952BAAD
CSB-EP730952BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S13 (rpsM) CSB-YP730953BAAD
CSB-EP730953BAAD
CSB-BP730953BAAD
CSB-MP730953BAAD
CSB-EP730953BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S8 (rpsH) CSB-YP730954BAAD
CSB-EP730954BAAD
CSB-BP730954BAAD
CSB-MP730954BAAD
CSB-EP730954BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S10 (rpsJ) CSB-YP730955BAAD
CSB-EP730955BAAD
CSB-BP730955BAAD
CSB-MP730955BAAD
CSB-EP730955BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L1 (rplA) CSB-YP730956BAAD
CSB-EP730956BAAD
CSB-BP730956BAAD
CSB-MP730956BAAD
CSB-EP730956BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Lysine--tRNA ligase (lysS) CSB-YP730957BAAD
CSB-EP730957BAAD
CSB-BP730957BAAD
CSB-MP730957BAAD
CSB-EP730957BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0213 protein BCE33L0031 (BCE33L0031) CSB-YP730958BAAD
CSB-EP730958BAAD
CSB-BP730958BAAD
CSB-MP730958BAAD
CSB-EP730958BAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein with KRAB and SCAN domains 2 (ZKSCAN2), partial CSB-YP730959HU
CSB-EP730959HU
CSB-BP730959HU
CSB-MP730959HU
CSB-EP730959HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pecanex-like protein C14orf135 (C14orf135), partial CSB-YP730960HU
CSB-EP730960HU
CSB-BP730960HU
CSB-MP730960HU
CSB-EP730960HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia pseudomallei ATP synthase subunit beta 2 (atpD2), partial CSB-YP730961BXW
CSB-EP730961BXW
CSB-BP730961BXW
CSB-MP730961BXW
CSB-EP730961BXW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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