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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant NADH-quinone oxidoreductase subunit I 1 (nuoI1) CSB-YP731523SRW
CSB-EP731523SRW
CSB-BP731523SRW
CSB-MP731523SRW
CSB-EP731523SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP731524SRW
CSB-EP731524SRW
CSB-BP731524SRW
CSB-MP731524SRW
CSB-EP731524SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP731525SRW
CSB-EP731525SRW
CSB-BP731525SRW
CSB-MP731525SRW
CSB-EP731525SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L19 (rplS) CSB-YP731526SRW
CSB-EP731526SRW
CSB-BP731526SRW
CSB-MP731526SRW
CSB-EP731526SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L32 (rpmF) CSB-YP731527SRW
CSB-EP731527SRW
CSB-BP731527SRW
CSB-MP731527SRW
CSB-EP731527SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L28 (rpmB) CSB-YP731528SRW
CSB-EP731528SRW
CSB-BP731528SRW
CSB-MP731528SRW
CSB-EP731528SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0296 protein STH1338 (STH1338) CSB-YP731529SRW
CSB-EP731529SRW
CSB-BP731529SRW
CSB-MP731529SRW
CSB-EP731529SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase 2 (murB2) CSB-YP731530SRW
CSB-EP731530SRW
CSB-BP731530SRW
CSB-MP731530SRW
CSB-EP731530SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Queuine tRNA-ribosyltransferase (tgt) CSB-YP731531SRW
CSB-EP731531SRW
CSB-BP731531SRW
CSB-MP731531SRW
CSB-EP731531SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP731532SRW
CSB-EP731532SRW
CSB-BP731532SRW
CSB-MP731532SRW
CSB-EP731532SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin--NADP reductase (STH965) CSB-YP731533SRW
CSB-EP731533SRW
CSB-BP731533SRW
CSB-MP731533SRW
CSB-EP731533SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP731534SRW
CSB-EP731534SRW
CSB-BP731534SRW
CSB-MP731534SRW
CSB-EP731534SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein CrcB homolog 1 (crcB1), partial CSB-YP731535SRW1
CSB-EP731535SRW1
CSB-BP731535SRW1
CSB-MP731535SRW1
CSB-EP731535SRW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP731536SRW
CSB-EP731536SRW
CSB-BP731536SRW
CSB-MP731536SRW
CSB-EP731536SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L21 (rplU) CSB-YP731537SRW
CSB-EP731537SRW
CSB-BP731537SRW
CSB-MP731537SRW
CSB-EP731537SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable GTP-binding protein EngB (engB) CSB-YP731538SRW
CSB-EP731538SRW
CSB-BP731538SRW
CSB-MP731538SRW
CSB-EP731538SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionine import ATP-binding protein MetN (metN) CSB-YP731539SRW
CSB-EP731539SRW
CSB-BP731539SRW
CSB-MP731539SRW
CSB-EP731539SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0042 nucleotide-binding protein STH186 (STH186) CSB-YP731540SRW
CSB-EP731540SRW
CSB-BP731540SRW
CSB-MP731540SRW
CSB-EP731540SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP731541SRW
CSB-EP731541SRW
CSB-BP731541SRW
CSB-MP731541SRW
CSB-EP731541SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uracil phosphoribosyltransferase (upp) CSB-YP731542SRW
CSB-EP731542SRW
CSB-BP731542SRW
CSB-MP731542SRW
CSB-EP731542SRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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