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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse InaD-like protein (Inadl), partial CSB-YP731022MO
CSB-EP731022MO
CSB-BP731022MO
CSB-MP731022MO
CSB-EP731022MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis GATS-like protein 1 (gatsl1) CSB-YP731023XBF
CSB-EP731023XBF
CSB-BP731023XBF
CSB-MP731023XBF
CSB-EP731023XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Transcription factor E3 (Tfe3) CSB-YP731024MO
CSB-EP731024MO
CSB-BP731024MO
CSB-MP731024MO
CSB-EP731024MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SHC SH2 domain-binding protein 1 homolog B (shcbp1-b), partial CSB-YP731025XBE
CSB-EP731025XBE
CSB-BP731025XBE
CSB-MP731025XBE
CSB-EP731025XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Dihydropyrimidinase-related protein 3-B (dpysl3-b), partial CSB-YP731026XBE
CSB-EP731026XBE
CSB-BP731026XBE
CSB-MP731026XBE
CSB-EP731026XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Glycerophosphodiester phosphodiesterase domain-containing protein 5 (Gdpd5), partial CSB-YP731027MO1
CSB-EP731027MO1
CSB-BP731027MO1
CSB-MP731027MO1
CSB-EP731027MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Glycosyltransferase 8 domain-containing protein 2 (Glt8d2), partial CSB-YP731028MO
CSB-EP731028MO
CSB-BP731028MO
CSB-MP731028MO
CSB-EP731028MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis WD repeat domain phosphoinositide-interacting protein 3 (wdr45l) CSB-YP731029XBF
CSB-EP731029XBF
CSB-BP731029XBF
CSB-MP731029XBF
CSB-EP731029XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meriones unguiculatus Alpha-1-antitrypsin CSB-YP731030MRA
CSB-EP731030MRA
CSB-BP731030MRA
CSB-MP731030MRA
CSB-EP731030MRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Lithocholate 6-beta-hydroxylase (CYP3A10), partial CSB-YP731031MRG
CSB-EP731031MRG
CSB-BP731031MRG
CSB-MP731031MRG
CSB-EP731031MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Lysosomal acid lipase/cholesteryl ester hydrolase (Lipa) CSB-YP731032RA
CSB-MP731032RA
CSB-EP731032RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Lipase member H-B (liph-b) CSB-YP731033XBE
CSB-EP731033XBE
CSB-BP731033XBE
CSB-MP731033XBE
CSB-EP731033XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytosolic carboxypeptidase 1 (Agtpbp1), partial CSB-YP731034MO
CSB-EP731034MO
CSB-BP731034MO
CSB-MP731034MO
CSB-EP731034MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Nuclear fragile X mental retardation-interacting protein 1 (Nufip1) CSB-YP731036RA
CSB-EP731036RA
CSB-BP731036RA
CSB-MP731036RA
CSB-EP731036RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Homeobox protein DLX-2 (Dlx2) CSB-YP731037RA
CSB-EP731037RA
CSB-BP731037RA
CSB-MP731037RA
CSB-EP731037RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Elongation factor 1-beta (ef1b) CSB-YP731038FPM
CSB-EP731038FPM
CSB-BP731038FPM
CSB-MP731038FPM
CSB-EP731038FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat ADP-ribosyl cyclase 1 (Cd38), partial CSB-YP731039RA
CSB-EP731039RA
CSB-BP731039RA
CSB-MP731039RA
CSB-EP731039RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse DNA repair protein complementing XP-A cells homolog (Xpa) CSB-YP731040MO
CSB-EP731040MO
CSB-BP731040MO
CSB-MP731040MO
CSB-EP731040MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Leukocyte immunoglobulin-like receptor subfamily B member 4 (Lilrb4), partial CSB-YP731041MO
CSB-EP731041MO
CSB-BP731041MO
CSB-MP731041MO
CSB-EP731041MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Neurogenic differentiation factor 1 (Neurod1) CSB-YP731042RA
CSB-EP731042RA
CSB-BP731042RA
CSB-MP731042RA
CSB-EP731042RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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