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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Adenylosuccinate synthetase isozyme 1 (adssl1) CSB-YP732244XBF
CSB-EP732244XBF
CSB-BP732244XBF
CSB-MP732244XBF
CSB-EP732244XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein C19orf12 homolog, partial CSB-YP732245XBF1
CSB-EP732245XBF1
CSB-BP732245XBF1
CSB-MP732245XBF1
CSB-EP732245XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Probable cysteine--tRNA ligase, mitochondrial (cars2), partial CSB-YP732246XBF
CSB-EP732246XBF
CSB-BP732246XBF
CSB-MP732246XBF
CSB-EP732246XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cleavage and polyadenylation specificity factor subunit 5 (cpsf5) CSB-YP732247XBE
CSB-EP732247XBE
CSB-BP732247XBE
CSB-MP732247XBE
CSB-EP732247XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Zinc finger CCHC-type and RNA-binding motif-containing protein 1 (zcrb1) CSB-YP732248XBE
CSB-EP732248XBE
CSB-BP732248XBE
CSB-MP732248XBE
CSB-EP732248XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis TNF receptor-associated factor 6-B (traf6-b), partial CSB-YP732249XBE
CSB-EP732249XBE
CSB-BP732249XBE
CSB-MP732249XBE
CSB-EP732249XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis NEDD4-binding protein 1 (n4bp1), partial CSB-YP732250XBF
CSB-EP732250XBF
CSB-BP732250XBF
CSB-MP732250XBF
CSB-EP732250XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cornifelin homolog B (cnfn-b) CSB-YP732251XBE
CSB-EP732251XBE
CSB-BP732251XBE
CSB-MP732251XBE
CSB-EP732251XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein CWC15 homolog B (cwc15-b) CSB-YP732252XBE
CSB-EP732252XBE
CSB-BP732252XBE
CSB-MP732252XBE
CSB-EP732252XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Envelope glycoprotein H (gH), partial CSB-YP732253EFJ
CSB-EP732253EFJ
CSB-BP732253EFJ
CSB-MP732253EFJ
CSB-EP732253EFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Macrophage migration inhibitory factor (MIF) CSB-YP732254MOW
CSB-EP732254MOW
CSB-BP732254MOW
CSB-MP732254MOW
CSB-EP732254MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrus psorosis virus Uncharacterized 54 kDa protein (54K) CSB-YP732255CFAZ
CSB-EP732255CFAZ
CSB-BP732255CFAZ
CSB-MP732255CFAZ
CSB-EP732255CFAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Soluble guanylate cyclase gcy-36 (gcy-36), partial CSB-YP732256CXY
CSB-EP732256CXY
CSB-BP732256CXY
CSB-MP732256CXY
CSB-EP732256CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP732257IBM
CSB-EP732257IBM
CSB-BP732257IBM
CSB-MP732257IBM
CSB-EP732257IBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP732258IBL
CSB-EP732258IBL
CSB-BP732258IBL
CSB-MP732258IBL
CSB-EP732258IBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP732259IBW
CSB-EP732259IBW
CSB-BP732259IBW
CSB-MP732259IBW
CSB-EP732259IBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP732260IGJ
CSB-EP732260IGJ
CSB-BP732260IGJ
CSB-MP732260IGJ
CSB-EP732260IGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP732261IEL
CSB-EP732261IEL
CSB-BP732261IEL
CSB-MP732261IEL
CSB-EP732261IEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP732262IGJ
CSB-EP732262IGJ
CSB-BP732262IGJ
CSB-MP732262IGJ
CSB-EP732262IGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 2 (M), partial CSB-YP732263IBQ1
CSB-EP732263IBQ1
CSB-BP732263IBQ1
CSB-MP732263IBQ1
CSB-EP732263IBQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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