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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Transcription factor bHLH130 (BHLH130) CSB-YP731423DOA
CSB-EP731423DOA
CSB-BP731423DOA
CSB-MP731423DOA
CSB-EP731423DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat F-box only protein 39 (Fbxo39) CSB-YP731424RA
CSB-EP731424RA
CSB-BP731424RA
CSB-MP731424RA
CSB-EP731424RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Polypyrimidine tract-binding protein 2 (Ptbp2) CSB-YP731425RA
CSB-EP731425RA
CSB-BP731425RA
CSB-MP731425RA
CSB-EP731425RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C9orf40 homolog CSB-YP731426RA
CSB-EP731426RA
CSB-BP731426RA
CSB-MP731426RA
CSB-EP731426RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Ly6/PLAUR domain-containing protein 1 (Lypd1) CSB-YP731427RA
CSB-EP731427RA
CSB-BP731427RA
CSB-MP731427RA
CSB-EP731427RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Uncharacterized protein C12orf71 homolog CSB-YP731428RA
CSB-EP731428RA
CSB-BP731428RA
CSB-MP731428RA
CSB-EP731428RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat RNA-binding protein 47 (Rbm47) CSB-YP731429RA
CSB-EP731429RA
CSB-BP731429RA
CSB-MP731429RA
CSB-EP731429RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Presqualene diphosphate phosphatase (Ppapdc2), partial CSB-YP731430RA1
CSB-EP731430RA1
CSB-BP731430RA1
CSB-MP731430RA1
CSB-EP731430RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Tax1-binding protein 1 homolog (Tax1bp1), partial CSB-YP731431RA
CSB-EP731431RA
CSB-BP731431RA
CSB-MP731431RA
CSB-EP731431RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Radial spoke head 10 homolog B (Rsph10b), partial CSB-YP731432RA
CSB-EP731432RA
CSB-BP731432RA
CSB-MP731432RA
CSB-EP731432RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein FAM221B (Fam221b) CSB-YP731433RA
CSB-EP731433RA
CSB-BP731433RA
CSB-MP731433RA
CSB-EP731433RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat NUAK family SNF1-like kinase 2 (Nuak2) CSB-YP731434RA
CSB-EP731434RA
CSB-BP731434RA
CSB-MP731434RA
CSB-EP731434RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Leucine-rich repeat flightless-interacting protein 1 (Lrrfip1) CSB-YP731435RA
CSB-EP731435RA
CSB-BP731435RA
CSB-MP731435RA
CSB-EP731435RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Solute carrier family 12 member 9 (Slc12a9), partial CSB-YP731436RA
CSB-EP731436RA
CSB-BP731436RA
CSB-MP731436RA
CSB-EP731436RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat PDZ and LIM domain protein 3 (Pdlim3) CSB-YP731437RA
CSB-EP731437RA
CSB-BP731437RA
CSB-MP731437RA
CSB-EP731437RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Pyridoxal-dependent decarboxylase domain-containing protein 1 (pdxdc1), partial CSB-YP731438DIL
CSB-EP731438DIL
CSB-BP731438DIL
CSB-MP731438DIL
CSB-EP731438DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Argininosuccinate synthase (ass1) CSB-YP731439DIL
CSB-EP731439DIL
CSB-BP731439DIL
CSB-MP731439DIL
CSB-EP731439DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Intraflagellar transport protein 20 homolog (ift20) CSB-YP731440DIL
CSB-EP731440DIL
CSB-BP731440DIL
CSB-MP731440DIL
CSB-EP731440DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Chitinase domain-containing protein 1 (chid1) CSB-YP731441XBF
CSB-EP731441XBF
CSB-BP731441XBF
CSB-MP731441XBF
CSB-EP731441XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM70A (fam70a), partial CSB-YP731442XBE1
CSB-EP731442XBE1
CSB-BP731442XBE1
CSB-MP731442XBE1
CSB-EP731442XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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