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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi A Thiamine-phosphate synthase (thiE) CSB-YP732454SBM
CSB-EP732454SBM
CSB-BP732454SBM
CSB-MP732454SBM
CSB-EP732454SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Porin ompL (ompL) CSB-YP732455SBM
CSB-EP732455SBM
CSB-BP732455SBM
CSB-MP732455SBM
CSB-EP732455SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A HTH-type transcriptional activator rhaS (rhaS) CSB-YP732456SBM
CSB-EP732456SBM
CSB-BP732456SBM
CSB-MP732456SBM
CSB-EP732456SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A L-rhamnose-proton symporter (rhaT), partial CSB-YP732457SBM
CSB-EP732457SBM
CSB-BP732457SBM
CSB-MP732457SBM
CSB-EP732457SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Porphobilinogen deaminase (hemC) CSB-YP732458SBM
CSB-EP732458SBM
CSB-BP732458SBM
CSB-MP732458SBM
CSB-EP732458SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP732459SBM
CSB-EP732459SBM
CSB-BP732459SBM
CSB-MP732459SBM
CSB-EP732459SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Membrane protein insertase YidC (yidC), partial CSB-YP732460SBM1
CSB-EP732460SBM1
CSB-BP732460SBM1
CSB-MP732460SBM1
CSB-EP732460SBM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Chromosomal replication initiator protein DnaA (dnaA) CSB-YP732461SBM
CSB-EP732461SBM
CSB-BP732461SBM
CSB-MP732461SBM
CSB-EP732461SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP732462SBM
CSB-EP732462SBM
CSB-BP732462SBM
CSB-MP732462SBM
CSB-EP732462SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A ATP synthase subunit b (atpF), partial CSB-YP732463SBM1
CSB-EP732463SBM1
CSB-BP732463SBM1
CSB-MP732463SBM1
CSB-EP732463SBM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Maltoporin (lamB) CSB-YP732464SBM
CSB-EP732464SBM
CSB-BP732464SBM
CSB-MP732464SBM
CSB-EP732464SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Chorismate--pyruvate lyase (ubiC) CSB-YP732465SBM
CSB-EP732465SBM
CSB-BP732465SBM
CSB-MP732465SBM
CSB-EP732465SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A tRNA dimethylallyltransferase (miaA) CSB-YP732466SBM
CSB-EP732466SBM
CSB-BP732466SBM
CSB-MP732466SBM
CSB-EP732466SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Elongation factor P (efp) CSB-YP732467SBM
CSB-EP732467SBM
CSB-BP732467SBM
CSB-MP732467SBM
CSB-EP732467SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0213 protein yhbQ (yhbQ) CSB-YP732468SBM
CSB-EP732468SBM
CSB-BP732468SBM
CSB-MP732468SBM
CSB-EP732468SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Phosphoglucosamine mutase (glmM) CSB-YP732469SBM
CSB-EP732469SBM
CSB-BP732469SBM
CSB-MP732469SBM
CSB-EP732469SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Putative mannosyl-3-phosphoglycerate phosphatase (yedP) CSB-YP732470SBM
CSB-EP732470SBM
CSB-BP732470SBM
CSB-MP732470SBM
CSB-EP732470SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP732471SBM
CSB-EP732471SBM
CSB-BP732471SBM
CSB-MP732471SBM
CSB-EP732471SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A UPF0370 protein ypfN (ypfN), partial CSB-YP732472SBM
CSB-EP732472SBM
CSB-BP732472SBM
CSB-MP732472SBM
CSB-EP732472SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi A Fe/S biogenesis protein NfuA (nfuA) CSB-YP732473SBM
CSB-EP732473SBM
CSB-BP732473SBM
CSB-MP732473SBM
CSB-EP732473SBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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