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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Danio rerio Major facilitator superfamily domain-containing protein 5 (mfsd5), partial CSB-YP732224DIL
CSB-EP732224DIL
CSB-BP732224DIL
CSB-MP732224DIL
CSB-EP732224DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ubiquitin-conjugating enzyme E2 Z (ube2z) CSB-YP732225DIL
CSB-EP732225DIL
CSB-BP732225DIL
CSB-MP732225DIL
CSB-EP732225DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein unc-45 homolog B (unc45b), partial CSB-YP732226DIL
CSB-EP732226DIL
CSB-BP732226DIL
CSB-MP732226DIL
CSB-EP732226DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein orthopedia B (otpb) CSB-YP732227DIL
CSB-EP732227DIL
CSB-BP732227DIL
CSB-MP732227DIL
CSB-EP732227DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Transcription factor Sox-1a (sox1a) CSB-YP732228DIL
CSB-EP732228DIL
CSB-BP732228DIL
CSB-MP732228DIL
CSB-EP732228DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Pre-mRNA-splicing factor syf2 (syf2) CSB-YP732229DIL
CSB-EP732229DIL
CSB-BP732229DIL
CSB-MP732229DIL
CSB-EP732229DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Non-structural maintenance of chromosomes element 1 homolog (nsmce1) CSB-YP732230DIL
CSB-EP732230DIL
CSB-BP732230DIL
CSB-MP732230DIL
CSB-EP732230DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Coiled-coil domain-containing protein 75 (ccdc75) CSB-YP732231DIL
CSB-EP732231DIL
CSB-BP732231DIL
CSB-MP732231DIL
CSB-EP732231DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio WD repeat domain-containing protein 83 (wdr83) CSB-YP732232DIL
CSB-EP732232DIL
CSB-BP732232DIL
CSB-MP732232DIL
CSB-EP732232DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Cytochrome c oxidase protein 20 homolog (cox20), partial CSB-YP732233DIL1
CSB-EP732233DIL1
CSB-BP732233DIL1
CSB-MP732233DIL1
CSB-EP732233DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Spindle and kinetochore-associated protein 1 (ska1) CSB-YP732234DIL
CSB-EP732234DIL
CSB-BP732234DIL
CSB-MP732234DIL
CSB-EP732234DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Coiled-coil domain-containing protein 85C-B (ccdc85cb) CSB-YP732235DIL
CSB-EP732235DIL
CSB-BP732235DIL
CSB-MP732235DIL
CSB-EP732235DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Ras-GEF domain-containing family member 1B-A (rasgef1ba), partial CSB-YP732236DIL
CSB-EP732236DIL
CSB-BP732236DIL
CSB-MP732236DIL
CSB-EP732236DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human TBC1 domain family member 3G (TBC1D3G) CSB-YP732237HU
CSB-EP732237HU
CSB-BP732237HU
CSB-MP732237HU
CSB-EP732237HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Uncharacterized protein C19orf68 homolog CSB-YP732238MO
CSB-EP732238MO
CSB-BP732238MO
CSB-MP732238MO
CSB-EP732238MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Multiple epidermal growth factor-like domains protein 10 (Megf10), partial CSB-YP732239MO
CSB-EP732239MO
CSB-BP732239MO
CSB-MP732239MO
CSB-EP732239MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Serine/arginine-rich splicing factor 1 (srsf1) CSB-YP732240XBF
CSB-EP732240XBF
CSB-BP732240XBF
CSB-MP732240XBF
CSB-EP732240XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Tubulin delta chain (tubd1) CSB-YP732241XBF
CSB-EP732241XBF
CSB-BP732241XBF
CSB-MP732241XBF
CSB-EP732241XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Echinoderm microtubule-associated protein-like 4 (eml4), partial CSB-YP732242XBF
CSB-EP732242XBF
CSB-BP732242XBF
CSB-MP732242XBF
CSB-EP732242XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Leucine zipper putative tumor suppressor 2 homolog (lzts2), partial CSB-YP732243XBF
CSB-EP732243XBF
CSB-BP732243XBF
CSB-MP732243XBF
CSB-EP732243XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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