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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Human Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 7 (AGAP7) CSB-YP733157HU
CSB-EP733157HU
CSB-BP733157HU
CSB-MP733157HU
CSB-EP733157HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human OTU domain-containing protein 1 (OTUD1) CSB-YP733158HU
CSB-EP733158HU
CSB-BP733158HU
CSB-MP733158HU
CSB-EP733158HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Peroxisome proliferator-activated receptor gamma coactivator-related protein 1 (PPRC1), partial CSB-YP733159HU
CSB-EP733159HU
CSB-BP733159HU
CSB-MP733159HU
CSB-EP733159HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DDB1- and CUL4-associated factor 12-like protein 2 (DCAF12L2) CSB-YP733160HU
CSB-EP733160HU
CSB-BP733160HU
CSB-MP733160HU
CSB-EP733160HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transcription elongation regulator 1-like protein (TCERG1L) CSB-YP733161HU
CSB-EP733161HU
CSB-BP733161HU
CSB-MP733161HU
CSB-EP733161HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Interleukin-23 receptor (IL23R), partial CSB-YP733162HU
CSB-EP733162HU
CSB-BP733162HU
CSB-MP733162HU
CSB-EP733162HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PRAME family member 9/15 (PRAMEF9) CSB-YP733163HU
CSB-EP733163HU
CSB-BP733163HU
CSB-MP733163HU
CSB-EP733163HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sushi domain-containing protein 4 (SUSD4), partial CSB-YP733164HU
CSB-EP733164HU
CSB-BP733164HU
CSB-MP733164HU
CSB-EP733164HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 2 (NKAIN2), partial CSB-YP733165HU1
CSB-EP733165HU1
CSB-BP733165HU1
CSB-MP733165HU1
CSB-EP733165HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein FAM75A3 (FAM75A3), partial CSB-YP733166HU
CSB-EP733166HU
CSB-BP733166HU
CSB-MP733166HU
CSB-EP733166HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tudor domain-containing protein 10 (TDRD10) CSB-YP733167HU
CSB-EP733167HU
CSB-BP733167HU
CSB-MP733167HU
CSB-EP733167HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DENN domain-containing protein 4C (DENND4C), partial CSB-YP733168HU
CSB-EP733168HU
CSB-BP733168HU
CSB-MP733168HU
CSB-EP733168HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Methyltransferase-like protein 21C (METTL21C) CSB-YP733169HU
CSB-EP733169HU
CSB-BP733169HU
CSB-MP733169HU
CSB-EP733169HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C10orf136 (C10orf136) CSB-YP733170HU
CSB-EP733170HU
CSB-BP733170HU
CSB-MP733170HU
CSB-EP733170HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Forkhead box protein P1 (foxp1), partial CSB-YP733171XBE
CSB-EP733171XBE
CSB-BP733171XBE
CSB-MP733171XBE
CSB-EP733171XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Folliculin-interacting protein 1 (FNIP1), partial CSB-YP733172CH
CSB-EP733172CH
CSB-BP733172CH
CSB-MP733172CH
CSB-EP733172CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative mannan endo-1,4-beta-mannosidase 5 (MAN5) CSB-YP733173OFG
CSB-EP733173OFG
CSB-BP733173OFG
CSB-MP733173OFG
CSB-EP733173OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Putative WUSCHEL-related homeobox 2 (WOX2) CSB-YP733174OFG
CSB-EP733174OFG
CSB-BP733174OFG
CSB-MP733174OFG
CSB-EP733174OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum UDP-sugar pyrophospharylase (USP), partial CSB-YP733175EWE
CSB-EP733175EWE
CSB-BP733175EWE
CSB-MP733175EWE
CSB-EP733175EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus clausii 50S ribosomal protein L25 (rplY) CSB-YP733176BAAC
CSB-EP733176BAAC
CSB-BP733176BAAC
CSB-MP733176BAAC
CSB-EP733176BAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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