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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Idiomarina loihiensis Lipoprotein-releasing system ATP-binding protein LolD (lolD) CSB-YP732534IAAA
CSB-EP732534IAAA
CSB-BP732534IAAA
CSB-MP732534IAAA
CSB-EP732534IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP732535IAAA
CSB-EP732535IAAA
CSB-BP732535IAAA
CSB-MP732535IAAA
CSB-EP732535IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP732536IAAA
CSB-EP732536IAAA
CSB-BP732536IAAA
CSB-MP732536IAAA
CSB-EP732536IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP732537IAAA
CSB-EP732537IAAA
CSB-BP732537IAAA
CSB-MP732537IAAA
CSB-EP732537IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L19 (rplS) CSB-YP732538IAAA
CSB-EP732538IAAA
CSB-BP732538IAAA
CSB-MP732538IAAA
CSB-EP732538IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis D-tyrosyl-tRNA (Tyr) deacylase (dtd) CSB-YP732539IAAA
CSB-EP732539IAAA
CSB-BP732539IAAA
CSB-MP732539IAAA
CSB-EP732539IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Imidazolonepropionase (hutI) CSB-YP732540IAAA
CSB-EP732540IAAA
CSB-BP732540IAAA
CSB-MP732540IAAA
CSB-EP732540IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Ribose-5-phosphate isomerase A (rpiA) CSB-YP732541IAAA
CSB-EP732541IAAA
CSB-BP732541IAAA
CSB-MP732541IAAA
CSB-EP732541IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Queuine tRNA-ribosyltransferase (tgt) CSB-YP732542IAAA
CSB-EP732542IAAA
CSB-BP732542IAAA
CSB-MP732542IAAA
CSB-EP732542IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis S-adenosylmethionine synthase (metK) CSB-YP732543IAAA
CSB-EP732543IAAA
CSB-BP732543IAAA
CSB-MP732543IAAA
CSB-EP732543IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Iron-sulfur cluster insertion protein ErpA (erpA) CSB-YP732544IAAA
CSB-EP732544IAAA
CSB-BP732544IAAA
CSB-MP732544IAAA
CSB-EP732544IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis tRNA (uracil (54)-C (5))-methyltransferase (trmA) CSB-YP732545IAAA
CSB-EP732545IAAA
CSB-BP732545IAAA
CSB-MP732545IAAA
CSB-EP732545IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Protein SlyX homolog (slyX) CSB-YP732546IAAA
CSB-EP732546IAAA
CSB-BP732546IAAA
CSB-MP732546IAAA
CSB-EP732546IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Protein syd (syd) CSB-YP732547IAAA
CSB-EP732547IAAA
CSB-BP732547IAAA
CSB-MP732547IAAA
CSB-EP732547IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis tRNA dimethylallyltransferase (miaA) CSB-YP732548IAAA
CSB-EP732548IAAA
CSB-BP732548IAAA
CSB-MP732548IAAA
CSB-EP732548IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 50S ribosomal protein L7/L12 (rplL) CSB-YP732549IAAA
CSB-EP732549IAAA
CSB-BP732549IAAA
CSB-MP732549IAAA
CSB-EP732549IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis 30S ribosomal protein S12 (rpsL) CSB-YP732550IAAA
CSB-EP732550IAAA
CSB-BP732550IAAA
CSB-MP732550IAAA
CSB-EP732550IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis N-succinylarginine dihydrolase (astB) CSB-YP732551IAAA
CSB-EP732551IAAA
CSB-BP732551IAAA
CSB-MP732551IAAA
CSB-EP732551IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis Recombination protein RecR (recR) CSB-YP732552IAAA
CSB-EP732552IAAA
CSB-BP732552IAAA
CSB-MP732552IAAA
CSB-EP732552IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Idiomarina loihiensis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP732553IAAA
CSB-EP732553IAAA
CSB-BP732553IAAA
CSB-MP732553IAAA
CSB-EP732553IAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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