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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia typhi 50S ribosomal protein L7/L12 (rplL) CSB-YP734720RNE
CSB-EP734720RNE
CSB-BP734720RNE
CSB-MP734720RNE
CSB-EP734720RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Putative glutamine transport system permease protein glnP (glnP), partial CSB-YP734721RNE1
CSB-EP734721RNE1
CSB-BP734721RNE1
CSB-MP734721RNE1
CSB-EP734721RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi ATP synthase subunit c (atpE), partial CSB-YP734722RNE1
CSB-EP734722RNE1
CSB-BP734722RNE1
CSB-MP734722RNE1
CSB-EP734722RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 30S ribosomal protein S18 (rpsR) CSB-YP734723RNE
CSB-EP734723RNE
CSB-BP734723RNE
CSB-MP734723RNE
CSB-EP734723RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Uncharacterized lipoprotein RT0080 (RT0080) CSB-YP734724RNE
CSB-EP734724RNE
CSB-BP734724RNE
CSB-MP734724RNE
CSB-EP734724RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Arginine--tRNA ligase (argS), partial CSB-YP734725RNE
CSB-EP734725RNE
CSB-BP734725RNE
CSB-MP734725RNE
CSB-EP734725RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Protein-export membrane protein SecG (secG), partial CSB-YP734726RNE1
CSB-EP734726RNE1
CSB-BP734726RNE1
CSB-MP734726RNE1
CSB-EP734726RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Probable ABC transporter permease protein RT0041 (RT0041), partial CSB-YP734727RNE1
CSB-BP734727RNE1
CSB-MP734727RNE1
CSB-EP734727RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Uncharacterized protein RT0029 (RT0029), partial CSB-YP734728RNE
CSB-EP734728RNE
CSB-BP734728RNE
CSB-MP734728RNE
CSB-EP734728RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Ribonuclease 3 (rnc) CSB-YP734729RNE
CSB-EP734729RNE
CSB-BP734729RNE
CSB-MP734729RNE
CSB-EP734729RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi UvrABC system protein A (uvrA), partial CSB-YP734730RNE
CSB-EP734730RNE
CSB-BP734730RNE
CSB-MP734730RNE
CSB-EP734730RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Tubby-like F-box protein 9 (TULP9) CSB-YP734731OFG
CSB-EP734731OFG
CSB-BP734731OFG
CSB-MP734731OFG
CSB-EP734731OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sapporo virus Uncharacterized protein VP3 (ORF3) CSB-YP734732SFAG
CSB-EP734732SFAG
CSB-BP734732SFAG
CSB-MP734732SFAG
CSB-EP734732SFAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6B Protein B7 (B7) CSB-YP734733HKA
CSB-EP734733HKA
CSB-BP734733HKA
CSB-MP734733HKA
CSB-EP734733HKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Tetratricopeptide repeat protein GNN (Gnn), partial CSB-YP734734MO
CSB-EP734734MO
CSB-BP734734MO
CSB-MP734734MO
CSB-EP734734MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glossina morsitans morsitans Superoxide dismutase [Mn], mitochondrial (Sod2) CSB-YP734735GGM
CSB-EP734735GGM
CSB-BP734735GGM
CSB-MP734735GGM
CSB-EP734735GGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla DNA dC->dU-editing enzyme APOBEC-3G (APOBEC3G) CSB-YP734736GGZ
CSB-EP734736GGZ
CSB-BP734736GGZ
CSB-MP734736GGZ
CSB-EP734736GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Phosphoprotein 85 (U14), partial CSB-YP734737HJZ
CSB-EP734737HJZ
CSB-BP734737HJZ
CSB-MP734737HJZ
CSB-EP734737HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Glycoprotein U23 (U23), partial CSB-YP734738HJZ1
CSB-EP734738HJZ1
CSB-BP734738HJZ1
CSB-MP734738HJZ1
CSB-EP734738HJZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Glycoprotein Q1 (U100) CSB-YP734739HJZ
CSB-EP734739HJZ
CSB-BP734739HJZ
CSB-MP734739HJZ
CSB-EP734739HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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