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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rickettsia typhi Ribosome-binding factor A (rbfA) CSB-YP737933RNE
CSB-EP737933RNE
CSB-BP737933RNE
CSB-MP737933RNE
CSB-EP737933RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Diaminopimelate epimerase (dapF) CSB-YP737934RNE
CSB-EP737934RNE
CSB-BP737934RNE
CSB-MP737934RNE
CSB-EP737934RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Putative peptidyl-prolyl cis-trans isomerase RT0383 (RT0383) CSB-YP737935RNE
CSB-EP737935RNE
CSB-BP737935RNE
CSB-MP737935RNE
CSB-EP737935RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP737936RNE
CSB-EP737936RNE
CSB-BP737936RNE
CSB-MP737936RNE
CSB-EP737936RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 30S ribosomal protein S4 (rpsD) CSB-YP737937RNE
CSB-EP737937RNE
CSB-BP737937RNE
CSB-MP737937RNE
CSB-EP737937RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi ATP-dependent protease subunit HslV (hslV) CSB-YP737938RNE
CSB-EP737938RNE
CSB-BP737938RNE
CSB-MP737938RNE
CSB-EP737938RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Uncharacterized protein RT0286 (RT0286) CSB-YP737939RNE
CSB-EP737939RNE
CSB-BP737939RNE
CSB-MP737939RNE
CSB-EP737939RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP737940RNE
CSB-EP737940RNE
CSB-BP737940RNE
CSB-MP737940RNE
CSB-EP737940RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP737941RNE
CSB-EP737941RNE
CSB-BP737941RNE
CSB-MP737941RNE
CSB-EP737941RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP737942RNE
CSB-EP737942RNE
CSB-BP737942RNE
CSB-MP737942RNE
CSB-EP737942RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Co-chaperone protein hscB homolog (hscB) CSB-YP737943RNE
CSB-EP737943RNE
CSB-BP737943RNE
CSB-MP737943RNE
CSB-EP737943RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Signal recognition particle protein (ffh) CSB-YP737944RNE
CSB-EP737944RNE
CSB-BP737944RNE
CSB-MP737944RNE
CSB-EP737944RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) CSB-YP737945RNE
CSB-EP737945RNE
CSB-BP737945RNE
CSB-MP737945RNE
CSB-EP737945RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 50S ribosomal protein L11 (rplK) CSB-YP737946RNE
CSB-EP737946RNE
CSB-BP737946RNE
CSB-MP737946RNE
CSB-EP737946RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Succinate dehydrogenase cytochrome b556 subunit (sdhC), partial CSB-YP737947RNE1
CSB-EP737947RNE1
CSB-BP737947RNE1
CSB-MP737947RNE1
CSB-EP737947RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi UPF0301 protein RT0098 (RT0098) CSB-YP737948RNE
CSB-EP737948RNE
CSB-BP737948RNE
CSB-MP737948RNE
CSB-EP737948RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP737949RNE
CSB-EP737949RNE
CSB-BP737949RNE
CSB-MP737949RNE
CSB-EP737949RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP737950RNE
CSB-EP737950RNE
CSB-BP737950RNE
CSB-MP737950RNE
CSB-EP737950RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP737951RNE
CSB-EP737951RNE
CSB-BP737951RNE
CSB-MP737951RNE
CSB-EP737951RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 3-deoxy-D-manno-octulosonic-acid transferase (waaA), partial CSB-YP737952RNE
CSB-EP737952RNE
CSB-BP737952RNE
CSB-MP737952RNE
CSB-EP737952RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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