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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Lysine-specific demethylase 2B (kdm2b), partial CSB-YP737331XBE
CSB-EP737331XBE
CSB-BP737331XBE
CSB-MP737331XBE
CSB-EP737331XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cell cycle progression protein 1 (Ccpg1), partial CSB-YP737332MO
CSB-EP737332MO
CSB-BP737332MO
CSB-MP737332MO
CSB-EP737332MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Acyl-CoA-binding domain-containing protein 5 (acbd5), partial CSB-YP737333XBF1
CSB-EP737333XBF1
CSB-BP737333XBF1
CSB-MP737333XBF1
CSB-EP737333XBF1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Nuclear receptor subfamily 2 group E member 1 (Nr2e1) CSB-YP737334MO
CSB-EP737334MO
CSB-BP737334MO
CSB-MP737334MO
CSB-EP737334MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Myosin regulatory light polypeptide 9 (Myl9) CSB-YP737335RA
CSB-EP737335RA
CSB-BP737335RA
CSB-MP737335RA
CSB-EP737335RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat T-cell surface glycoprotein CD3 gamma chain (Cd3g), partial CSB-YP737336RA
CSB-EP737336RA
CSB-BP737336RA
CSB-MP737336RA
CSB-EP737336RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Poly (A) RNA polymerase GLD2-A (papd4-a), partial CSB-YP737337XBE
CSB-EP737337XBE
CSB-BP737337XBE
CSB-MP737337XBE
CSB-EP737337XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Acyl-CoA-binding domain-containing protein 5 (acbd5), partial CSB-YP737338XBE1
CSB-EP737338XBE1
CSB-BP737338XBE1
CSB-MP737338XBE1
CSB-EP737338XBE1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase RNF12-A (rnf12-a), partial CSB-YP737339XBE
CSB-EP737339XBE
CSB-BP737339XBE
CSB-MP737339XBE
CSB-EP737339XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse NUAK family SNF1-like kinase 1 (Nuak1) CSB-YP737340MO
CSB-EP737340MO
CSB-BP737340MO
CSB-MP737340MO
CSB-EP737340MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat RNA polymerase-associated protein LEO1 (Leo1) CSB-YP737341RA
CSB-EP737341RA
CSB-BP737341RA
CSB-MP737341RA
CSB-EP737341RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein phosphatase 1J (Ppm1j) CSB-YP737342RA
CSB-EP737342RA
CSB-BP737342RA
CSB-MP737342RA
CSB-EP737342RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cation transport regulator-like protein 2 (Chac2) CSB-YP737343RA
CSB-EP737343RA
CSB-BP737343RA
CSB-MP737343RA
CSB-EP737343RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Homeobox protein OTX2 (Otx2) CSB-YP737344RA
CSB-EP737344RA
CSB-BP737344RA
CSB-MP737344RA
CSB-EP737344RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Alpha-sarcoglycan (SGCA), partial CSB-YP737345MRG
CSB-EP737345MRG
CSB-BP737345MRG
CSB-MP737345MRG
CSB-EP737345MRG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Inward rectifier potassium channel 2 (Kcnj2), partial CSB-YP737346RA1
CSB-EP737346RA1
CSB-BP737346RA1
CSB-MP737346RA1
CSB-EP737346RA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Transmembrane protein ENSP00000340100 homolog, partial CSB-YP737347RA1
CSB-EP737347RA1
CSB-BP737347RA1
CSB-MP737347RA1
CSB-EP737347RA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein FAM151A (Fam151a), partial CSB-YP737348RA1
CSB-EP737348RA1
CSB-BP737348RA1
CSB-MP737348RA1
CSB-EP737348RA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Synembryn-A (ric8a), partial CSB-YP737349DIL
CSB-EP737349DIL
CSB-BP737349DIL
CSB-MP737349DIL
CSB-EP737349DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (dhdh) CSB-YP737350DIL
CSB-EP737350DIL
CSB-BP737350DIL
CSB-MP737350DIL
CSB-EP737350DIL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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