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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kluyveromyces lactis Autophagy-related protein 8 (ATG8) CSB-YP738353KBK
CSB-EP738353KBK
CSB-BP738353KBK
CSB-MP738353KBK
CSB-EP738353KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Biogenesis of lysosome-related organelles complex 1 subunit KXD1 (KXD1) CSB-YP738354KBK
CSB-EP738354KBK
CSB-BP738354KBK
CSB-MP738354KBK
CSB-EP738354KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Histone H2B.2 (HTB1) CSB-YP738355KBK
CSB-EP738355KBK
CSB-BP738355KBK
CSB-MP738355KBK
CSB-EP738355KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Altered inheritance of mitochondria protein 23, mitochondrial (AIM23) CSB-YP738356KBK
CSB-EP738356KBK
CSB-BP738356KBK
CSB-MP738356KBK
CSB-EP738356KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 60S ribosomal subunit assembly/export protein LOC1 (LOC1) CSB-YP738357KBK
CSB-EP738357KBK
CSB-BP738357KBK
CSB-MP738357KBK
CSB-EP738357KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Phosphatidylglycerol/phosphatidylinositol transfer protein (NPC2) CSB-YP738358KBK
CSB-EP738358KBK
CSB-BP738358KBK
CSB-MP738358KBK
CSB-EP738358KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Heterogeneous nuclear rnp K-like protein 2 (HEK2) CSB-YP738359KBK
CSB-EP738359KBK
CSB-BP738359KBK
CSB-MP738359KBK
CSB-EP738359KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mediator of RNA polymerase II transcription subunit 3 (PGD1) CSB-YP738360KBK
CSB-EP738360KBK
CSB-BP738360KBK
CSB-MP738360KBK
CSB-EP738360KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mitochondrial import inner membrane translocase subunit TIM14 (PAM18), partial CSB-YP738361KBK1
CSB-EP738361KBK1
CSB-BP738361KBK1
CSB-MP738361KBK1
CSB-EP738361KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP738362KBK
CSB-EP738362KBK
CSB-BP738362KBK
CSB-MP738362KBK
CSB-EP738362KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis High osmolarity signaling protein SHO1 (SHO1), partial CSB-YP738363KBK1
CSB-EP738363KBK1
CSB-BP738363KBK1
CSB-MP738363KBK1
CSB-EP738363KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Bud site selection protein 4 (BUD4), partial CSB-YP738364KBK
CSB-EP738364KBK
CSB-BP738364KBK
CSB-MP738364KBK
CSB-EP738364KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Pre-mRNA-splicing factor SYF2 (SYF2) CSB-YP738365KBK
CSB-EP738365KBK
CSB-BP738365KBK
CSB-MP738365KBK
CSB-EP738365KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein transport protein SEC61 subunit alpha (SEC61), partial CSB-YP738366KBK
CSB-EP738366KBK
CSB-BP738366KBK
CSB-MP738366KBK
CSB-EP738366KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein SQS1 (SQS1), partial CSB-YP738367KBK
CSB-EP738367KBK
CSB-BP738367KBK
CSB-MP738367KBK
CSB-EP738367KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Serine/threonine-protein phosphatase 4 regulatory subunit 3 (PSY2), partial CSB-YP738368KBK
CSB-EP738368KBK
CSB-BP738368KBK
CSB-MP738368KBK
CSB-EP738368KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis COP9 signalosome complex subunit 9 (CSN9) CSB-YP738369KBK
CSB-EP738369KBK
CSB-BP738369KBK
CSB-MP738369KBK
CSB-EP738369KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Tethering factor for nuclear proteasome STS1 (STS1) CSB-YP738370KBK
CSB-EP738370KBK
CSB-BP738370KBK
CSB-MP738370KBK
CSB-EP738370KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Glutamyl-tRNA (Gln) amidotransferase subunit F, mitochondrial (KLLA0D15246g) CSB-YP738371KBK
CSB-EP738371KBK
CSB-BP738371KBK
CSB-MP738371KBK
CSB-EP738371KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein FMP52, mitochondrial (FMP52) CSB-YP738372KBK
CSB-EP738372KBK
CSB-BP738372KBK
CSB-MP738372KBK
CSB-EP738372KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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