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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yarrowia lipolytica Histone acetyltransferase type B subunit 2 (HAT2) CSB-YP738293YAP
CSB-EP738293YAP
CSB-BP738293YAP
CSB-MP738293YAP
CSB-EP738293YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica FACT complex subunit POB3 (POB3), partial CSB-YP738294YAP
CSB-EP738294YAP
CSB-BP738294YAP
CSB-MP738294YAP
CSB-EP738294YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica ATP-dependent RNA helicase DBP7 (DBP7), partial CSB-YP738295YAP
CSB-EP738295YAP
CSB-BP738295YAP
CSB-MP738295YAP
CSB-EP738295YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica DNA ligase 4 (LIG4), partial CSB-YP738296YAP
CSB-EP738296YAP
CSB-BP738296YAP
CSB-MP738296YAP
CSB-EP738296YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Sterol 3-beta-glucosyltransferase (ATG26), partial CSB-YP738297YAP
CSB-EP738297YAP
CSB-BP738297YAP
CSB-MP738297YAP
CSB-EP738297YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica mRNA 3'-end-processing protein YTH1 (YTH1) CSB-YP738298YAP
CSB-EP738298YAP
CSB-BP738298YAP
CSB-MP738298YAP
CSB-EP738298YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica SWR1-complex protein 4 (SWC4), partial CSB-YP738299YAP
CSB-EP738299YAP
CSB-BP738299YAP
CSB-MP738299YAP
CSB-EP738299YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 13 (SSN2), partial CSB-YP738300YAP
CSB-EP738300YAP
CSB-BP738300YAP
CSB-MP738300YAP
CSB-EP738300YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 14 (RGR1), partial CSB-YP738301YAP
CSB-EP738301YAP
CSB-BP738301YAP
CSB-MP738301YAP
CSB-EP738301YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Helicase SWR1 (SWR1), partial CSB-YP738302YAP
CSB-EP738302YAP
CSB-BP738302YAP
CSB-MP738302YAP
CSB-EP738302YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Eukaryotic translation initiation factor 3 subunit L (YALI0D03399g), partial CSB-YP738303YAP
CSB-EP738303YAP
CSB-BP738303YAP
CSB-MP738303YAP
CSB-EP738303YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 31 (SOH1) CSB-YP738304YAP
CSB-EP738304YAP
CSB-BP738304YAP
CSB-MP738304YAP
CSB-EP738304YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mitochondrial division protein 1 (MDV1), partial CSB-YP738305YAP
CSB-EP738305YAP
CSB-BP738305YAP
CSB-MP738305YAP
CSB-EP738305YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica F-actin-capping protein subunit beta (CAP2) CSB-YP738306YAP
CSB-EP738306YAP
CSB-BP738306YAP
CSB-MP738306YAP
CSB-EP738306YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Adenylyltransferase and sulfurtransferase UBA4 (UBA4) CSB-YP738307YAP
CSB-EP738307YAP
CSB-BP738307YAP
CSB-MP738307YAP
CSB-EP738307YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica mRNA cap guanine-N7 methyltransferase (ABD1), partial CSB-YP738308YAP
CSB-EP738308YAP
CSB-BP738308YAP
CSB-MP738308YAP
CSB-EP738308YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Serine/threonine-protein kinase SSN3 (SSN3) CSB-YP738309YAP
CSB-EP738309YAP
CSB-BP738309YAP
CSB-MP738309YAP
CSB-EP738309YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Fe-S cluster assembly protein DRE2 (DRE2) CSB-YP738310YAP
CSB-EP738310YAP
CSB-BP738310YAP
CSB-MP738310YAP
CSB-EP738310YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Probable zinc metalloprotease YALI0C01133g (YALI0C01133g), partial CSB-YP738311YAP
CSB-EP738311YAP
CSB-BP738311YAP
CSB-MP738311YAP
CSB-EP738311YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica NAD (P)H-hydrate epimerase (YALI0B23122g) CSB-YP738312YAP
CSB-EP738312YAP
CSB-BP738312YAP
CSB-MP738312YAP
CSB-EP738312YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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