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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Candida glabrata Multiprotein-bridging factor 1 (MBF1) CSB-YP739155CZI
CSB-EP739155CZI
CSB-BP739155CZI
CSB-MP739155CZI
CSB-EP739155CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Actin-related protein 4 (ARP4) CSB-YP739156CZI
CSB-EP739156CZI
CSB-BP739156CZI
CSB-MP739156CZI
CSB-EP739156CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial holo-[acyl-carrier-protein] synthase (PPT2) CSB-YP739157CZI
CSB-EP739157CZI
CSB-BP739157CZI
CSB-MP739157CZI
CSB-EP739157CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Probable zinc metalloprotease CAGL0M01936g (CAGL0M01936g), partial CSB-YP739158CZI
CSB-EP739158CZI
CSB-BP739158CZI
CSB-MP739158CZI
CSB-EP739158CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Damage-regulated import facilitator 1 (DIF1) CSB-YP739159CZI
CSB-EP739159CZI
CSB-BP739159CZI
CSB-MP739159CZI
CSB-EP739159CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein transport protein BOS1 (BOS1), partial CSB-YP739160CZI
CSB-EP739160CZI
CSB-BP739160CZI
CSB-MP739160CZI
CSB-EP739160CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata SAGA-associated factor 11 (SGF11) CSB-YP739161CZI
CSB-EP739161CZI
CSB-BP739161CZI
CSB-MP739161CZI
CSB-EP739161CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Genetic interactor of prohibitin 5, mitochondrial (GEP5) CSB-YP739162CZI
CSB-EP739162CZI
CSB-BP739162CZI
CSB-MP739162CZI
CSB-EP739162CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Cap-associated protein CAF20 (CAF20) CSB-YP739163CZI
CSB-EP739163CZI
CSB-BP739163CZI
CSB-MP739163CZI
CSB-EP739163CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Enhancer of translation termination 1 (ETT1) CSB-YP739164CZI
CSB-EP739164CZI
CSB-BP739164CZI
CSB-MP739164CZI
CSB-EP739164CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata PAB-dependent poly (A)-specific ribonuclease subunit PAN3 (PAN3), partial CSB-YP739165CZI
CSB-EP739165CZI
CSB-BP739165CZI
CSB-MP739165CZI
CSB-EP739165CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Probable metalloprotease ARX1 (ARX1), partial CSB-YP739166CZI
CSB-EP739166CZI
CSB-BP739166CZI
CSB-MP739166CZI
CSB-EP739166CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DASH complex subunit DAM1 (DAM1) CSB-YP739167CZI
CSB-EP739167CZI
CSB-BP739167CZI
CSB-MP739167CZI
CSB-EP739167CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata ATP-dependent RNA helicase DBP2 (DBP2), partial CSB-YP739168CZI
CSB-EP739168CZI
CSB-BP739168CZI
CSB-MP739168CZI
CSB-EP739168CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 (KAE1) CSB-YP739169CZI
CSB-EP739169CZI
CSB-BP739169CZI
CSB-MP739169CZI
CSB-EP739169CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mannan endo-1,6-alpha-mannosidase DCW1 (DCW1) CSB-YP739170CZI
CSB-EP739170CZI
CSB-BP739170CZI
CSB-MP739170CZI
CSB-EP739170CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pre-mRNA-splicing factor RSE1 (RSE1), partial CSB-YP739171CZI
CSB-EP739171CZI
CSB-BP739171CZI
CSB-MP739171CZI
CSB-EP739171CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Calcineurin subunit B (CNB1) CSB-YP739172CZI
CSB-EP739172CZI
CSB-BP739172CZI
CSB-MP739172CZI
CSB-EP739172CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Hsp70 nucleotide exchange factor FES1 (FES1) CSB-YP739173CZI
CSB-EP739173CZI
CSB-BP739173CZI
CSB-MP739173CZI
CSB-EP739173CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 37S ribosomal protein S10, mitochondrial (RSM10) CSB-YP739174CZI
CSB-EP739174CZI
CSB-BP739174CZI
CSB-MP739174CZI
CSB-EP739174CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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