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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter sp. 30S ribosomal protein S17 (rpsQ) CSB-YP739235AWW
CSB-EP739235AWW
CSB-BP739235AWW
CSB-MP739235AWW
CSB-EP739235AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L18 (rplR) CSB-YP739236AWW
CSB-EP739236AWW
CSB-BP739236AWW
CSB-MP739236AWW
CSB-EP739236AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 30S ribosomal protein S11 (rpsK) CSB-YP739237AWW
CSB-EP739237AWW
CSB-BP739237AWW
CSB-MP739237AWW
CSB-EP739237AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Malate dehydrogenase (mdh) CSB-YP739238AWW
CSB-EP739238AWW
CSB-BP739238AWW
CSB-MP739238AWW
CSB-EP739238AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Peptide chain release factor 3 (prfC), partial CSB-YP739239AWW
CSB-EP739239AWW
CSB-BP739239AWW
CSB-MP739239AWW
CSB-EP739239AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Integration host factor subunit alpha (ihfA) CSB-YP739240AWW
CSB-EP739240AWW
CSB-BP739240AWW
CSB-MP739240AWW
CSB-EP739240AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Serine--tRNA ligase (serS) CSB-YP739241AWW
CSB-EP739241AWW
CSB-BP739241AWW
CSB-MP739241AWW
CSB-EP739241AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Peptidyl-tRNA hydrolase (pth) CSB-YP739242AWW
CSB-EP739242AWW
CSB-BP739242AWW
CSB-MP739242AWW
CSB-EP739242AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Carbamoyl-phosphate synthase small chain (carA) CSB-YP739243AWW
CSB-EP739243AWW
CSB-BP739243AWW
CSB-MP739243AWW
CSB-EP739243AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. UPF0178 protein ACIAD2644 (ACIAD2644) CSB-YP739244AWW
CSB-EP739244AWW
CSB-BP739244AWW
CSB-MP739244AWW
CSB-EP739244AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Elongation factor 4 (lepA), partial CSB-YP739245AWW
CSB-EP739245AWW
CSB-BP739245AWW
CSB-MP739245AWW
CSB-EP739245AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Coenzyme PQQ synthesis protein E (pqqE) CSB-YP739246AWW
CSB-EP739246AWW
CSB-BP739246AWW
CSB-MP739246AWW
CSB-EP739246AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Probable chorismate--pyruvate lyase (ubiC) CSB-YP739247AWW
CSB-EP739247AWW
CSB-BP739247AWW
CSB-MP739247AWW
CSB-EP739247AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L9 (rplI) CSB-YP739248AWW
CSB-EP739248AWW
CSB-BP739248AWW
CSB-MP739248AWW
CSB-EP739248AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP739249AWW
CSB-EP739249AWW
CSB-BP739249AWW
CSB-MP739249AWW
CSB-EP739249AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 30S ribosomal protein S2 (rpsB) CSB-YP739250AWW
CSB-EP739250AWW
CSB-BP739250AWW
CSB-MP739250AWW
CSB-EP739250AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. C4-dicarboxylate transport protein (dctA), partial CSB-YP739251AWW
CSB-EP739251AWW
CSB-BP739251AWW
CSB-MP739251AWW
CSB-EP739251AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. S-adenosylmethionine synthase (metK) CSB-YP739252AWW
CSB-EP739252AWW
CSB-BP739252AWW
CSB-MP739252AWW
CSB-EP739252AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Enolase (eno) CSB-YP739253AWW
CSB-EP739253AWW
CSB-BP739253AWW
CSB-MP739253AWW
CSB-EP739253AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 30S ribosomal protein S20 (rpsT) CSB-YP739254AWW
CSB-EP739254AWW
CSB-BP739254AWW
CSB-MP739254AWW
CSB-EP739254AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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